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Product Details
PA1-84986
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.1-0.2 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
Assay-dependent | - |
ELISA (ELISA) |
0.5-2.0 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant human Wnt-1 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
RRID |
AB_2241751 |
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections.
Reconstitute with 0.1 mL of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Rabbit anti Human WNT-1 antibody recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.
Target Information
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)
Gene Aliases: BMND16; INT1; OI15; WNT1
UniProt ID: (Human) P04628
Entrez Gene ID: (Human) 7471
Molecular Function:
intercellular signal molecule
embryonic axis specification
branching involved in ureteric bud morphogenesis
positive regulation of protein phosphorylation
cell-cell signaling
myoblast fusion
positive regulation of cell proliferation
response to wounding
positive regulation of lamellipodium assembly
negative regulation of cell-substrate adhesion
Wnt signaling pathway
spinal cord association neuron differentiation
diencephalon development
central nervous system morphogenesis
cerebellum formation
forebrain anterior/posterior pattern specification
midbrain-hindbrain boundary maturation during brain development
negative regulation of cell-cell adhesion
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
ubiquitin-dependent SMAD protein catabolic process
midbrain development
organ regeneration
T cell differentiation in thymus
cell proliferation in midbrain
inner ear morphogenesis
signal transduction in response to DNA damage
positive regulation of insulin-like growth factor receptor signaling pathway
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
cell fate commitment
negative regulation of fat cell differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
neuron fate determination
neuron development
positive regulation of sequence-specific DNA binding transcription factor activity
Spemann organizer formation
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
bone development
positive regulation of dermatome development
hepatocyte differentiation
cellular response to peptide hormone stimulus
hematopoietic stem cell proliferation
positive regulation of canonical Wnt signaling pathway
negative regulation of cell aging
beta-catenin destruction complex disassembly
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
embryonic brain development
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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