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WNT11 Antibody (PA5-116126) in ICC/IF
Immunocytochemistry analysis of WNT11 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116126) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116126
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNT11(Accession O96014), corresponding to amino acid residues V274-R324.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900760
Product Specific Information
Antibody detects endogenous levels of total WNT11.
Target Information
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-11; wingless-related MMTV integration site 11; wingless-type MMTV integration site family, member 11
Gene Aliases: HWNT11; Wnt-11; WNT11
UniProt ID: (Human) O96014, (Mouse) P48615
Entrez Gene ID: (Human) 7481, (Rat) 140584, (Mouse) 22411
osteoblast differentiation
epithelial to mesenchymal transition
outflow tract morphogenesis
planar cell polarity pathway involved in axis elongation
protein phosphorylation
Wnt signaling pathway, calcium modulating pathway
positive regulation of gene expression
Wnt signaling pathway
neuron differentiation
bone mineralization
negative regulation of cell growth
adrenal gland development
positive regulation of cell migration
negative regulation of cell migration
response to nutrient levels
activation of protein kinase activity
positive regulation of transforming growth factor beta2 production
protein localization to cell surface
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of GTPase activity
cell fate commitment
maintenance of epithelial cell apical/basal polarity
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
paraxial mesoderm formation
notochord morphogenesis
embryonic skeletal system development
artery morphogenesis
positive regulation of stress fiber assembly
palate development
convergent extension involved in axis elongation
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
cloacal septation
ventricular septum morphogenesis
lung-associated mesenchyme development
negative regulation of cell death
ureteric bud morphogenesis
planar cell polarity pathway involved in gastrula mediolateral intercalation
negative regulation of cartilage development
somite development
neuroendocrine cell differentiation
bicellular tight junction assembly
cellular response to retinoic acid
mesonephric duct development
negative regulation of mesenchymal cell proliferation
positive regulation of protein kinase C signaling
negative regulation of canonical Wnt signaling pathway
negative regulation of fibroblast growth factor production
kidney development
cell differentiation
non-canonical Wnt signaling pathway
positive regulation of protein kinase activity
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
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