Invitrogen

WNT11 Polyclonal Antibody

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WNT11 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of WNT11 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a WNT11 polyclonal antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

WNT11 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of WNT11 in paraformaldehyde-fixed HeLa cells using a WNT11 polyclonal antibody (Product # PA5-21712) at a 1:200 dilution.

WNT11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of WNT11 in the secretion of paraffin-embedded mouse colon tissue using a WNT11 polyclonal antibody (Product # PA5-21712) at a dilution of 1:500.

WNT11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of WNT11 in paraffin-embedded lung SCC xenograft using a WNT11 polyclonal antibody (Product # PA5-21712) at a dilution of 1:100.

WNT11 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), U2OS (Lane 2), HeLa (Lane 3), HT-29 (Lane 4), HEK-293 (Lane 5), MCF7 (Lane 6), PC-3 (Lane 7) and A-431 (Lane 8). The blot was probed with Anti-WNT11 Polyclonal Antibody (Product # PA5-21712, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). A 40 kDa band corresponding to WNT11 was observed across the cell lines tested.

WNT11 Antibody in Western Blot (WB) — Western blot analysis of WNT11 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT11 Polyclonal Antibody (Product # PA5-21712) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

WNT11 Antibody in Western Blot (WB) — Western Blot analysis of WNT11 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT11 Polyclonal Antibody (Product # PA5-21712) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

-

Immunofluorescence (IF)

1:100-1:1000

-

Immunohistochemistry (IHC)

Assay Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

View 1 publication 1 publication

Western Blot (WB)

1:500-1:3000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID#O96014)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154198

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse heart.

Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (81%), Rabbit (97%), Chicken (85%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Protein Wnt-11; wingless-related MMTV integration site 11; wingless-type MMTV integration site family, member 11

Gene Aliases: HWNT11; Wnt-11; WNT11

UniProt ID: (Human) O96014, (Mouse) P48615

Entrez Gene ID: (Human) 7481, (Mouse) 22411

Molecular Function: signaling molecule

osteoblast differentiation epithelial to mesenchymal transition outflow tract morphogenesis planar cell polarity pathway involved in axis elongation protein phosphorylation Wnt signaling pathway, calcium modulating pathway positive regulation of gene expression Wnt signaling pathway neuron differentiation bone mineralization negative regulation of cell growth adrenal gland development positive regulation of cell migration negative regulation of cell migration response to nutrient levels activation of protein kinase activity positive regulation of transforming growth factor beta2 production protein localization to cell surface positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of GTPase activity cell fate commitment maintenance of epithelial cell apical/basal polarity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated paraxial mesoderm formation notochord morphogenesis embryonic skeletal system development artery morphogenesis positive regulation of stress fiber assembly palate development convergent extension involved in axis elongation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway cloacal septation ventricular septum morphogenesis lung-associated mesenchyme development negative regulation of cell death ureteric bud morphogenesis planar cell polarity pathway involved in gastrula mediolateral intercalation negative regulation of cartilage development somite development neuroendocrine cell differentiation bicellular tight junction assembly cellular response to retinoic acid mesonephric duct development negative regulation of mesenchymal cell proliferation positive regulation of protein kinase C signaling negative regulation of canonical Wnt signaling pathway negative regulation of fibroblast growth factor production kidney development signal transduction cell-cell signaling multicellular organism development organ morphogenesis non-canonical Wnt signaling pathway positive regulation of protein kinase activity

Process(es)

GTPase activator activity frizzled binding protein binding protein kinase activator activity transcription regulatory region DNA binding receptor binding