Invitrogen

WNT11 Polyclonal Antibody

2 Published Figures

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WNT11 Antibody in Immunocytochemistry (ICC/IF) — WNT11 Polyclonal Antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

WNT11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11 antibody (Product # PA5-21712) at 1:200 dilution.

WNT11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. WNT11 antibody (Product # PA5-21712) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

WNT11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11 (Product # PA5-21712) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

WNT11 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), U2OS (Lane 2), HeLa (Lane 3), HT-29 (Lane 4), HEK-293 (Lane 5), MCF7 (Lane 6), PC-3 (Lane 7) and A-431 (Lane 8). The blot was probed with Anti-WNT11 Polyclonal Antibody (Product # PA5-21712, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). A 40 kDa band corresponding to WNT11 was observed across the cell lines tested.

WNT11 Antibody in Western Blot (WB) — Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

WNT11 Antibody in Western Blot (WB) — Western blot analysis of WNT11 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT11 Polyclonal Antibody (Product # PA5-21712) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

WNT11 Antibody in Western Blot (WB) — Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

WNT11 Antibody in Western Blot (WB) — Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

WNT11 Antibody in Western Blot (WB) — Western Blot analysis of WNT11 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNT11 Polyclonal Antibody (Product # PA5-21712) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

WNT11 Antibody in Immunohistochemistry (IHC) — Published figure using WNT11 polyclonal antibody (Product # PA5-21712) in Immunohistochemistry

WNT11 Antibody in Western Blot (WB) — Figure 2 WNT11 heterozygous mutant cells with decreased WNT11 mRNA and protein levels as well as proliferation and mineralization. ( A ) Electropherogram showing the 32-bp deletion leading to a frameshift. fs * : frameshift. ( B ) RT-qPCR analysis of WNT11 mRNA expression in mutant versus control U2OS cells. Normalization was to GAPDH level as a housekeeping gene with ratio of one for control U2OS cells. Ctrl: wild-type U2OS cells, Mut: WNT11 mutant cells, FC: fold change. ( C ) Western blot analysis of WNT11 protein expression, confirming the heterozygosity. a-Tubulin was a control. ( D ) Alizarin red staining showed formation of mineralized nodules after osteogenic differentiation treatment with osteogenic media in control and WNT11 mutant cells. ( E ) Proliferation of control and WNT11 mutant cells ( n = 42). Data are mean +- SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Product Details

PA5-21712

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID#O96014)

Conjugate

Unconjugated

Form

Liquid

Concentration

1.56 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154198

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse heart.

Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (81%), Rabbit (97%), Chicken (85%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

WNT11, a new gene associated with early onset osteoporosis, is required for osteoblastogenesis.

Human molecular genetics

Caetano da Silva C,Edouard T,Fradin M,Aubert-Mucca M,Ricquebourg M,Raman R,Salles JP,Charon V,Guggenbuhl P,Muller M,Cohen-Solal M,Collet C

Published figure using WNT11 polyclonal antibody (Product # PA5-21712) in Western Blot

Thu May 19 00:00:00 EDT 2022

Amniotic stromal stem cell-loaded hydrogel repairs cardiac tissue in infarcted rat hearts via paracrine mediators.

Journal of tissue engineering and regenerative medicine

Khan K,Makhoul G,Yu B,Jalani G,Derish I,Rutman AK,Cerruti M,Schwertani A,Cecere R

PA5-21712 was used in Western Blotting to demonstrate the cardioprotective potential of a composite inserting human amniotic stromal mesenchymal stem cells (ASMCs) in a chitosan and hyaluronic acid (C/HA) based hydrogel in a rat MI model.

Tue Feb 01 00:00:00 EST 2022

16p11.2 transcription factor MAZ is a dosage-sensitive regulator of genitourinary development.

Proceedings of the National Academy of Sciences of the United States of America

Haller M,Au J,O'Neill M,Lamb DJ

Published figure using WNT11 polyclonal antibody (Product # PA5-21712) in Immunohistochemistry

Tue Feb 20 00:00:00 EST 2018

Role of Noncanonical Wnt Signaling Pathway in Human Aortic Valve Calcification.

Arteriosclerosis, thrombosis, and vascular biology

Albanese I,Yu B,Al-Kindi H,Barratt B,Ott L,Al-Refai M,de Varennes B,Shum-Tim D,Cerruti M,Gourgas O,Rhéaume E,Tardif JC,Schwertani A

PA521712 was used in immunohistochemistry - paraffin section and western blot to discuss the impact of Wnt ligands on aortic valve calcification

Wed Mar 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Protein Wnt-11; wingless-related MMTV integration site 11; wingless-type MMTV integration site family, member 11

Gene Aliases: HWNT11; Wnt-11; WNT11

UniProt ID: (Human) O96014, (Mouse) P48615

Entrez Gene ID: (Human) 7481, (Mouse) 22411

Molecular Function: intercellular signal molecule

osteoblast differentiation epithelial to mesenchymal transition outflow tract morphogenesis planar cell polarity pathway involved in axis elongation protein phosphorylation Wnt signaling pathway, calcium modulating pathway positive regulation of gene expression Wnt signaling pathway neuron differentiation bone mineralization negative regulation of cell growth adrenal gland development positive regulation of cell migration negative regulation of cell migration response to nutrient levels activation of protein kinase activity positive regulation of transforming growth factor beta2 production protein localization to cell surface positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of GTPase activity cell fate commitment maintenance of epithelial cell apical/basal polarity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated paraxial mesoderm formation notochord morphogenesis embryonic skeletal system development artery morphogenesis positive regulation of stress fiber assembly palate development convergent extension involved in axis elongation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway cloacal septation ventricular septum morphogenesis lung-associated mesenchyme development negative regulation of cell death ureteric bud morphogenesis planar cell polarity pathway involved in gastrula mediolateral intercalation negative regulation of cartilage development somite development neuroendocrine cell differentiation bicellular tight junction assembly cellular response to retinoic acid mesonephric duct development negative regulation of mesenchymal cell proliferation positive regulation of protein kinase C signaling negative regulation of canonical Wnt signaling pathway negative regulation of fibroblast growth factor production kidney development signal transduction cell-cell signaling multicellular organism development organ morphogenesis non-canonical Wnt signaling pathway positive regulation of protein kinase activity

Process(es)

GTPase activator activity frizzled binding protein binding protein kinase activator activity transcription regulatory region DNA binding receptor binding