Immunofluorescent analysis of WNT11 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a WNT11 polyclonal antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID#O96014)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21712 targets WNT11 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-21712 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID#O96014).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.