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Immunofluorescent staining of chick embryos using Rb anti-WNT6 (Cat. no. 38-3400). Antibody labels the non-neural ectoderm of chick embryos from stages 8 to 14, consistent with mRNA expression by ISH. Additional staining is observed in endodermal tissues caudal to the otic level in heart- and gut-forming regions. Image courtesy of Dr. Martin Garcia Castro, California Institute of Technology, Pasadena, CA.
|Tested species reactivity||Chicken|
|Published species reactivity||Clawed frog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the chicken WNT6 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Clawed frog||Not Cited||Wnt6 signaling regulates heart muscle development during organogenesis.||Lavery DL,Martin J,Turnbull YD,Hoppler S||Developmental biology (323:177)||2008|