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Agrisera
WUS/WUSCHEL protein Polyclonal Antibody
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FIGURE: 1 / 1
WUS/WUSCHEL protein Antibody (AS11-1759) in WB
Plant material: Col-0 wild type (1), p WUS: : WUS-GFP (2), expression level comparable to wild type p Ubiquitin: : m Cherry-GR-WUS (3), over-expresses the wuschel protein and also has ectopic expression. Used as a positive control for the westerns. 200 mg of 5-7 day old seedling were extracted with 600 µL of 1 x SDS-PAGE loading buffer (50 m M Tris-HCl p H 6.8,2% SDS, 10% glycerol, 1% β-Me SH, 12.5 m M EDTA, 0.02% bromophenol blue) supplemented with 1% Sigma Protease Inhibitor Cocktail (Cat. Num: P 9599). Protein amounts in the extracts we... View More
Product Details
AS11-1759
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana WUSCHEL protein sequence.
Conjugate
Form
Liquid
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
WUS encodes a plant-specific WUSCHEL-related homeobox transcription factor that localizes to the shoot apical meristem organizing center (niche) beneath the stem cells, and its protein can move from the organizing center into the adjacent central zone to execute non-cell-autonomous control of stem cell behavior. The gene produces a small, intron-containing transcript encoding an approximately 291-292 amino acid nuclear protein with a conserved N-terminal homeodomain responsible for sequence-specific DNA binding, plus a characteristic C-terminal WUS box required for its regulatory activities. Functionally, WUS is essential for establishing and maintaining the shoot stem cell niche: it promotes stem cell fate in overlying cells while simultaneously driving a homeostatic negative-feedback circuit by activating CLAVATA3 expression in neighboring stem cells, which in turn restricts WUS expression to prevent stem cell overproliferation; at the transcriptional level WUS also directly represses differentiation programs in meristematic progeny, thereby preventing premature organogenic differentiation and sustaining long-term meristem function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT2G17950; PGA6; T27K22.18; T27K22_18; WUS1; WUSCHEL; WUSCHEL 1
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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