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Wnt1 Polyclonal Antibody

View all (12) WNT1 antibodies

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Product Details

600-401-A37

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

ELISA (ELISA)

1:25,000
-

Immunoprecipitation (IP)

1:1,500-1:6,000
-
Product Specifications

Species Reactivity

Bovine, Dog, Human, Marsupial, Mouse, Rhesus monkey, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: murine mammary tumor virus integration site; Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1; WNT1

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UniProt ID: (Human) P04628, (Mouse) P04426

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Entrez Gene ID: (Human) 7471, (Dog) 486560, (Rhesus monkey) 709009, (Bovine) 540662, (Rat) 24881, (Mouse) 22408

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Function(s)
receptor binding frizzled binding cytokine activity morphogen activity protein domain specific binding receptor agonist activity molecular_function transcription regulatory region DNA binding protein binding intercellular signal molecule
Process(es)
embryonic axis specification branching involved in ureteric bud morphogenesis cell-cell signaling myoblast fusion cell proliferation positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion myotube differentiation Wnt signaling pathway spinal cord association neuron differentiation diencephalon development cerebellum development central nervous system morphogenesis cerebellum formation forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development neurogenesis metencephalon development negative regulation of cell-cell adhesion neuron differentiation negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway midbrain development midbrain-hindbrain boundary development organ regeneration T cell differentiation in thymus cell proliferation in midbrain astrocyte-dopaminergic neuron signaling inner ear morphogenesis signal transduction in response to DNA damage negative regulation of apoptotic process positive regulation of insulin-like growth factor receptor signaling pathway cell fate commitment fat cell differentiation negative regulation of cell differentiation negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation neuron fate commitment neuron fate determination Spemann organizer formation canonical Wnt signaling pathway bone development positive regulation of dermatome development hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation dopaminergic neuron differentiation positive regulation of hematopoietic stem cell proliferation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of cellular senescence positive regulation of protein phosphorylation ubiquitin-dependent SMAD protein catabolic process canonical Wnt signaling pathway involved in negative regulation of apoptotic process positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of cell aging signal transduction multicellular organism development organ morphogenesis BMP signaling pathway
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