Invitrogen

XDH Polyclonal Antibody

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XDH Antibody in Western Blot (WB) — Western blot analysis of extracts of mouse liver, using XDH Polyclonal antibody (Product # PA5-88946) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

-

Product Specifications

Species Reactivity

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XDH

Conjugate

Unconjugated

Form

liquid

Concentration

3.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2805243

Product Specific Information

Immunogen sequence: MTADKLVFFV NGRKVVEKNA DPETTLLAYL RRKLGLSGTK LGCGEGGCGA CTVMLSKYDR LQNKIVHFSA NACLAPICSL HHVAVTTVEG IGSTKTRLHP VQERIAKSHG SQCGFCTPGI VMSMYTLLRN QPEPTMEEIE NAFQGNLCRC TGYRPILQGF RTFARDGGCC GGDGNNPNCC MNQKKDHSVS LSPSLFKPEE FTPLDPTQEP IFPPELLRLK DTPRKQLRFE GERVTWIQAS TLKELLDLKA QHPDAKLVVG NTEIGIEMKF KNMLFPMIVC; Positive Samples: Mouse liver; Cellular Location: Cytoplasm, Peroxisome

Target Information

XDH belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. This protein can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XO; XOR

Gene Aliases: Xdh; XO; Xor; Xox-1; Xox1

UniProt ID: (Mouse) Q00519

Entrez Gene ID: (Mouse) 22436

Molecular Function: dehydrogenase oxidase oxidoreductase

negative regulation of protein phosphorylation negative regulation of endothelial cell proliferation activation of cysteine-type endopeptidase activity involved in apoptotic process lactation xanthine catabolic process response to aluminum ion negative regulation of gene expression regulation of epithelial cell differentiation bone resorption negative regulation of endothelial cell differentiation negative regulation of protein kinase B signaling oxidation-reduction process positive regulation of p38MAPK cascade negative regulation of vascular endothelial growth factor signaling pathway positive regulation of reactive oxygen species metabolic process negative regulation of vasculogenesis

Process(es)

catalytic activity xanthine dehydrogenase activity xanthine oxidase activity iron ion binding protein binding UDP-N-acetylmuramate dehydrogenase activity electron carrier activity oxidoreductase activity oxidoreductase activity, acting on CH-OH group of donors oxidoreductase activity, acting on the aldehyde or oxo group of donors molybdenum ion binding protein homodimerization activity molybdopterin cofactor binding metal ion binding flavin adenine dinucleotide binding iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding