Invitrogen

XRCC1 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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XRCC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of XRCC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with XRCC1 Polyclonal Antibody (Product # PA5-118362, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

XRCC1 Antibody in Western Blot (WB) — Western Blot using XRCC1 Polyclonal Antibody (Product # PA5-118362) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa.

XRCC1 Antibody in Immunoprecipitation (IP) — XRCC1 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with XRCC1 Polyclonal Antibody (Product # PA5-118362) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: XRCC1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 95 kDa.

Product Details

PA5-118362

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Immunoprecipitation (IP)	5-10 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human XRCC1 fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902956

Target Information

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein XRCC1; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1

Gene Aliases: RCC; XRCC1

UniProt ID: (Human) P18887

Entrez Gene ID: (Human) 7515

Molecular Function: DNA metabolism protein

Function(s)

single strand break repair double-strand break repair via homologous recombination response to hypoxia transcription-coupled nucleotide-excision repair base-excision repair base-excision repair, DNA ligation nucleotide-excision repair, DNA gap filling response to organic substance response to drug

Process(es)

damaged DNA binding DNA ligase activity protein binding enzyme binding

XRCC1 Polyclonal Antibody

Product Details

PA5-118362

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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