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YARS Antibody (PA5-121039) in WB
Western blot analysis of YARS in extracts of various cell lines. Samples were incubated with YARS Polyclonal antibody (Product # PA5-121039) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.49 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2914611
Product Specific Information
Positive test controls include: HeLa, SKOV3, Mouse brain, Mouse skeletal muscle, Rat brain. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA
Target Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: tyrosine tRNA ligase 1, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed; tyrosyl trna synthetase; tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; TyrRS
Gene Aliases: AL024047; AU018965; CMTDIC; TYRRS; YARS; YARS1; YRS; YTS
UniProt ID: (Human) P54577, (Mouse) Q91WQ3, (Rat) Q4KM49
Entrez Gene ID: (Human) 8565, (Mouse) 107271, (Rat) 313047
Molecular Function:
translational protein
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