Invitrogen

ZCCHC11 Polyclonal Antibody

View all (15) ZCCHC11 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)

FIGURE: 1 / 4

ZCCHC11 Antibody (PA5-100054) in WB

Western blot analysis of ZCCHC11 in various samples (Lane 1: hepg2 treated with blocking peptide, Lane 2: Hepg2, Lane 3: B16F10). Samples were incubated with ZCCHC11 polyclonal antibody (Product # PA5-100054).

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ZCCHC11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZCCHC11 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ZCCHC11 polyclonal antibody (Product # PA5-100054) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ZCCHC11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ZCCHC11 in human lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ZCCHC11 polyclonal antibody (Product # PA5-100054) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Product Details

PA5-100054

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ZCCHC11(Accession Q5TAX3), corresponding to amino acid residues P1138-G1159.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2815584

Target Information

WDR77 is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PAP associated domain containing 3; RP23-406B13.1; Terminal uridylyltransferase 4; TUTase 4; Zinc finger CCHC domain-containing protein 11; zinc finger, CCHC domain containing 11

Gene Aliases: 6030404K05Rik; 9230115F04Rik; KIAA0191; mKIAA0191; PAPD3; PPAPD3; TUT4; ZCCHC11

UniProt ID: (Human) Q5TAX3, (Mouse) B2RX14

Entrez Gene ID: (Human) 23318, (Mouse) 230594

Molecular Function: nucleotidyltransferase transferase metabolite interconversion enzyme