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Invitrogen
ZEB1 Polyclonal Antibody
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FIGURE: 1 / 12
ZEB1 Antibody (PA5-28221) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ZEB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 Polyclonal Antibody (Product # PA5-28221) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-28221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.13 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, 293T, HepG2, MDA-MB-231, Neuro2A, NIH-3T3, C2C12, ZEB1 shRNA-transfected HeLa.
Predicted reactivity: Mouse (91%), Rat (89%), Chicken (90%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; zinc finger homeodomain enhancer-binding protein
Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha
UniProt ID: (Human) P37275, (Mouse) Q64318
Entrez Gene ID: (Human) 6935, (Mouse) 21417
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
protein binding
transcription factor binding
zinc ion binding
E-box binding
nucleic acid binding
DNA binding
double-stranded DNA binding
sequence-specific DNA binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
pattern specification process
central nervous system development
cell proliferation
negative regulation of cell proliferation
regulation of mesenchymal cell proliferation
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of epithelial cell differentiation
regulation of T cell differentiation in thymus
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic camera-type eye morphogenesis
embryonic skeletal system morphogenesis
semicircular canal morphogenesis
regulation of smooth muscle cell differentiation
cartilage development
cellular response to amino acid stimulus
cochlea morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
nervous system development
cell differentiation
animal organ development
embryonic morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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