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Invitrogen
ZFHX3 Polyclonal Antibody
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FIGURE: 1 / 5
ZFHX3 Antibody (PA5-119848) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human stomach carcinoma tissue using ZFHX3 Polyclonal Antibody (Product # PA5-119848). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ZFHX3 antibody at a ... View More
Product Details
PA5-119848
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human ATBF1 aa 1,800-2,000.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Rat esophagus tissue, human colon tissue, human breast carcinoma tissue, human stomach carcinoma tissue.
Subcellular Location: Nucleus, Cytoplasm.
Western Blot: 1:500 for endogenous lysates (predicted); 1:5,000 for recombinant protein.
Target Information
Transcriptional repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. Down-regulates the MUC5AC promoter in gastric cancer. Miura Y., J. Biol. Chem. 270:26840-26848(1995). Morinaga T., Mol. Cell. Biol. 11:6041-6049(1991). Martin J., Nature 432:988-994(2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-fetoprotein enhancer binding protein; Alpha-fetoprotein enhancer-binding protein; AT motif-binding factor 1; AT-binding transcription factor 1; unnamed protein product; ZFH-3; zinc finger homeobox 3; Zinc finger homeobox protein 3; Zinc finger homeodomain protein 3
Gene Aliases: ATBF1; ATBT; ATFB8; C16orf47; RGD1560268; SCA4; ZFH-3; ZFHX3; ZNF927
UniProt ID: (Human) Q15911
Entrez Gene ID: (Human) 463, (Rat) 307829
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
enzyme binding
metal ion binding
molecular_function
sequence-specific DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
muscle organ development
circadian regulation of gene expression
negative regulation of myoblast differentiation
positive regulation of myoblast differentiation
regulation of neuron differentiation
positive regulation of cell adhesion
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to transforming growth factor beta
regulation of locomotor rhythm
cell cycle arrest
brain development
negative regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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