Immunofluorescent analysis of ZNF181 showing staining in the nucleus of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a ZNF181 polyclonal antibody (Product # PA5-31109) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 10 and 263 of Human ZNF181|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31109 targets ZNF181 in WB applications and shows reactivity with Human samples.
The PA5-31109 immunogen is recombinant protein fragment corresponding to a region within amino acids 10 and 263 of Human ZNF181.
Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See MIM 604749 for additional information on zinc finger proteins.
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