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Invitrogen
ZO-1 Polyclonal Antibody
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FIGURE: 1 / 98
ZO-1 Antibody (40-2200) in ICC/IF
Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 IFN Gamma (50 ng/mL for 24hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZO-1 Polyclonal Antibody (Product # 40-2200) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More
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.jpg?time=20220826?time=20220826 "ZO-1 Antibody (40-2200) in ICC/IF")
Product Details
40-2200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Bat,
Bovine,
Chicken,
Dog,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Xenopus,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide in the middle region of the ZO-1 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
40-2200 was used successfully in the immunofluorescence analysis of Zo-1 in MDCK cells.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; unnamed protein product; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Human) Q07157, (Dog) O97758, (Mouse) P39447, (Rat) A0A0G2K2P5
Entrez Gene ID: (Human) 7082, (Dog) 403752, (Mouse) 21872, (Rat) 292994
cell-cell junction assembly
positive regulation of cell proliferation
actin cytoskeleton organization
positive regulation of cell migration
actomyosin structure organization
adherens junction maintenance
maintenance of blood-brain barrier
ameloblast differentiation
negative regulation of apoptotic process
cell-cell junction organization
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
protein localization to adherens junction
establishment of endothelial intestinal barrier
cell-cell adhesion
protein localization to cell-cell junction
regulation of cell junction assembly
protein localization to bicellular tight junction
positive regulation of sprouting angiogenesis
positive regulation of maintenance of permeability of blood-brain barrier
positive regulation of cell-cell adhesion mediated by cadherin
regulation of bicellular tight junction assembly
bicellular tight junction assembly
cellular response to calcium ion
blastocyst formation
sensory perception of sound
response to lipopolysaccharide
response to drug
negative regulation of vascular permeability
response to ethanol
response to magnetism
cellular response to glucose stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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