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Western blot analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), A549 (Lane 2), CaCo2 (Lane 3), MCF7 (lane 4) and HT-29 (lane 5). The blots were probed with Anti-alpha Catenin Rabbit Polyclonal Antibody (Product# 711200, 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution). Two bands ~ 100 and 59 kDa corresponding to alpha Catenin was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
|Tested species reactivity||Chicken, Human, Mouse, Rat, Clawed frog|
|Published species reactivity||Dog, Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human alpha Catenin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. Mice that have a-catenin ablated in their surface epithelium demonstrate reduced hair growth, multinucleated cells, extensive proliferation and a general cancerous phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Proliferation, cell cycle exit, and onset of terminal differentiation in cultured keratinocytes: pre-programmed pathways in control of C-Myc and Notch1 prevail over extracellular calcium signals.||Kolly C,Suter MM,Müller EJ||The Journal of investigative dermatology (124:1014)||2005|
|E-cadherin suppression accelerates squamous cell carcinoma progression in three-dimensional, human tissue constructs.||Margulis A,Zhang W,Alt-Holland A,Crawford HC,Fusenig NE,Garlick JA||Cancer research (65:1783)||2005|
|Rat||Not Cited||Myofibroblast development is characterized by specific cell-cell adherens junctions.||Hinz B,Pittet P,Smith-Clerc J,Chaponnier C,Meister JJ||Molecular biology of the cell (15:4310)||2004|
102 kDa cadherin-associated protein; 102kDa; alpha E catenin; alpha E-catenin; alpha(E)-catenin; alpha-catenin; alpha-E-catenin; alphaE-catenin; cadherin associated protein; cadherin-associated protein; CAP102; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; catenin alpha-1; renal carcinoma antigen NY-REN-13
2010010M04Rik; AA517462; AI988031; CAP102; Catna1; CTNNA1; MDPT2