Invitrogen

alpha-II Spectrin Polyclonal Antibody

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alpha-II Spectrin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of alpha-II Spectrin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha-II Spectrin Polyclonal Antibody (Product # PA5-35383) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

alpha-II Spectrin Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Staurosporine (0.5 uM for 24 hours) (Lane 2), Jurkat (Lane 3), Jurkat treated with Staurosporine (0.5 uM for 24 hours) (Lane 4), HeLa (Lane 5), HeLa treated with Staurosporine (0.5 uM for 24 hours) (Lane 6) and U-87 MG (Lane 7). The blot was probed with Anti-alpha-II Spectrin Polyclonal Antibody (Product # PA5-35383, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 260 kDa band corresponding to intact alpha-II Spectrin was observed across the untreated lysates tested. Upon Staurosporine treatment, alpha-II Spectrin degrades into two major fragments at 160 and 120 kDa.

alpha-II Spectrin Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of alpha-II Spectrin using alpha-II Spectrin Polyclonal Antibody (Product # PA5-35383) shows degradation of intact protein into two major fragments in SH-SY5Y, Jurkat and HeLa cell lines upon Staurosporine treatment. {TM}

Product Details

PA5-35383

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000	-
Immunohistochemistry (IHC)	1:500	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:250	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin
Conjugate	Unconjugated
Form	Liquid
Storage buffer	whole serum
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2552693

Product Specific Information

This antibody contains enough material to conduct 10 mini-Western blots.

Target Information

SPTAN1 (spectrin alpha chain, non-erythrocytic 1) is a part of the spectrin family of filamentous cytoskeletal proteins. They function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. SPTAN1 has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Retinoic acid degradation shapes zonal development of vestibular organs and sensitivity to transient linear accelerations.

Nature communications

Ono K,Keller J,López Ramírez O,González Garrido A,Zobeiri OA,Chang HHV,Vijayakumar S,Ayiotis A,Duester G,Della Santina CC,Jones SM,Cullen KE,Eatock RA,Wu DK

PA5-35383 was used in Immunohistochemistry-wholemount to propose that the formation of highly specialised regions of the vestibular sensory epithelia with specfic functions in detecting head motions requires degradation of retinoic acid.

Thu Jan 02 00:00:00 EST 2020

Bioinformatics

Protein Aliases: (ALPHA)II-SPECTRIN; a--fodrin; alpha; alpha II spectrin; alpha-fodrin; Alpha-II spectrin; alpha-spectrin 2; alpha-spectrin 2, brain; brain; brain alpha-spectrin; FLJ44613; Fodrin alpha chain; inhibitory protein factor; noerythroid alpha-spectrin 2; Non erythrocytic spectrin alpha; non-erythrocytic 1 (alpha-fodrin); non-erythroid alpha chain; nonerythroid alpha-spectrin 2; Spectrin; Spectrin alpha; Spectrin alpha chain brain; spectrin alpha chain, brain; Spectrin alpha chain, non-erythrocytic 1; spectrin, non-erythroid alpha chain; Spectrin, non-erythroid alpha subunit

Gene Aliases: 2610027H02Rik; A2a; EIEE5; IPF; NEAS; Spna-2; Spna2; SPTA2; SPTAN1

UniProt ID: (Human) Q13813, (Rat) P16086

Entrez Gene ID: (Human) 6709, (Mouse) 20740, (Rat) 64159

Function(s)

MAPK cascade ER to Golgi vesicle-mediated transport axon guidance actin cytoskeleton reorganization positive regulation of GTPase activity actin filament capping cell-cell adhesion

Process(es)

actin binding Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton calcium ion binding protein binding calmodulin binding syntaxin binding protein complex binding protein N-terminus binding cadherin binding involved in cell-cell adhesion spectrin binding metal ion binding protein heterodimerization activity

alpha-II Spectrin Polyclonal Antibody

Product Details

PA5-35383

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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