|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified, recombinant rat alpha-internexin, expressed in E. coli.|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, human proteins run at ~68kDa, while rat and mouse run lower at ~66kDa. Minor bands at ~150kDa are probably covalent dimers, and bands at ~50kDa represent a-internexin breakdown products.
Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage. In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.