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Product Details
PA5-85271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Fruit fly,
Horse,
Cat,
Hamster,
Human,
Mouse,
Pig,
Rat,
Xenopus,
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.06 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2792414
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
Positive Control: 293T, A341, Jurkat, Raji, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, PC-12, Rat2, Rat brain, drosophila, Yeast lysate, Candida albicans (sc5314) whole cell lysate, Rice leaf, mouse aorta, H9C2, OKT3, mouse ileum tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue, human periodontal ligament.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; Cap Z; capZ beta; cytoplasmic 1; Cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1
Gene Aliases: ACTB; actin; Actx; Bact; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; XELAEV_18045052mg
UniProt ID: (Horse) P60708, (Human) P60709, (Pig) Q6QAQ1, (Chicken) P60706, (Dog) O18840, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975, (Xenopus) O93400
Entrez Gene ID: (Horse) 100033878, (Human) 60, (Pig) 414396, (Chicken) 396526, (Dog) 403580, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477, (Xenopus) 398459
Molecular Function:
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
postsynaptic actin cytoskeleton organization
axonogenesis
cell motility
retina development in camera-type eye
cellular response to electrical stimulus
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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