|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) beta-Amyloid peptide|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 6 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|ELISA (ELISA)||See 2 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 2 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Western Blot (WB)||See 7 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer and quote;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of betaAPP and helps to modulate the production of the amyloid beta peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Catalytically active tissue transglutaminase colocalises with Aß pathology in Alzheimer's disease mouse models.
71-5800 was used in immunohistochemistry - frozen section to study colocalization of A-beta pathology in Alzheimer's disease mouse models with catalytically active tissue transglutaminase
|Wilhelmus MM,de Jager M,Smit AB,van der Loo RJ,Drukarch B||Scientific reports (6:null)||2016|
Genetic modulation of soluble Aß rescues cognitive and synaptic impairment in a mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - frozen section to study the role of soluble mutant amyloid precursor protein on cognitive function and synaptic structure
|Fowler SW,Chiang AC,Savjani RR,Larson ME,Sherman MA,Schuler DR,Cirrito JR,Lesné SE,Jankowsky JL||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:7871)||2014|
Liraglutide can reverse memory impairment, synaptic loss and reduce plaque load in aged APP/PS1 mice, a model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - frozen section to test if Liraglutide has restorative effects in late-stage Alzheimer's disease in mice.
|McClean PL,Hölscher C||Neuropharmacology (76 Pt A:57)||2014|
A novel retro-inverso peptide inhibitor reduces amyloid deposition, oxidation and inflammation and stimulates neurogenesis in the APPswe/PS1¿E9 mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - frozen section to study the use of a retro-inverso peptide inhibitor to treat a murine model of Alzheimer's disease.
|Parthsarathy V,McClean PL,Hölscher C,Taylor M,Tinker C,Jones G,Kolosov O,Salvati E,Gregori M,Masserini M,Allsop D||PloS one (8:null)||2013|
Butyrylcholinesterase is associated with ß-amyloid plaques in the transgenic APPSWE/PSEN1dE9 mouse model of Alzheimer disease.
71-5800 was used in immunohistochemistry - frozen section to investigate the role of BuChE using the APPSWE/PSENdE9 transgenic mouse model.
|Darvesh S,Cash MK,Reid GA,Martin E,Mitnitski A,Geula C||Journal of neuropathology and experimental neurology (71:2)||2012|
The diabetes drug liraglutide prevents degenerative processes in a mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - frozen section to examine the effects of peripherally injected liraglutide in an Alzheimer mouse model.
|McClean PL,Parthsarathy V,Faivre E,Hölscher C||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:6587)||2011|
Prophylactic liraglutide treatment prevents amyloid plaque deposition, chronic inflammation and memory impairment in APP/PS1 mice.
71-5800 was used in immunohistochemistry to demonstrate that liraglutide has prophylactic properties in Alzheimer's disease models.
|McClean PL,Jalewa J,Hölscher C||Behavioural brain research (293:96)||2015|
Lixisenatide, a drug developed to treat type 2 diabetes, shows neuroprotective effects in a mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry (frozen) to demonstrate that GLP-1 receptor agonists are therapeutic for Alzheimer's disease.
|McClean PL,Hölscher C||Neuropharmacology (86:241)||2014|
Val(8)GLP-1 rescues synaptic plasticity and reduces dense core plaques in APP/PS1 mice.
71-5800 was used in immunohistochemistry - frozen section to examine the effects of Val(8)GLP-1 in a mouse model of Alzheimer's disease
|Gengler S,McClean PL,McCurtin R,Gault VA,Hölscher C||Neurobiology of aging (33:265)||2012|
Sex differences in ?-amyloid accumulation in 3xTg-AD mice: role of neonatal sex steroid hormone exposure.
71-5800 was used in immunohistochemistry to explore the effects of sex steroid hormones in a mouse model of Alzheimer's disease
|Carroll JC,Rosario ER,Kreimer S,Villamagna A,Gentzschein E,Stanczyk FZ,Pike CJ||Brain research (1366:233)||2010|
Synaptic plasticity in the hippocampus of a APP/PS1 mouse model of Alzheimer's disease is impaired in old but not young mice.
71-5800 was used in immunohistochemistry to study synaptic plasticity in area CA1 in a novel Alzheimer disease mouse model
|Gengler S,Hamilton A,H?lscher C||PloS one (5:null)||2010|
Tissue transglutaminase-catalysed cross-linking induces Apolipoprotein E multimers inhibiting Apolipoprotein E's protective effects towards amyloid-beta-induced toxicity.
71-5800 was used in immunohistochemistry to study the role of apolipoprotein E in cerebral amyloid angiopathy.
|de Jager M,Drukarch B,Hofstee M,Brevé J,Jongenelen CA,Bol JG,Wilhelmus MM||Journal of neurochemistry (134:1116)||2015|
Butyrylcholinesterase-knockout reduces brain deposition of fibrillar ß-amyloid in an Alzheimer mouse model.
71-5800 was used in immunohistochemistry to investigate butyrylcholinesterase activity in a mouse model of Alzheimer's disease.
|Reid GA,Darvesh S||Neuroscience (298:424)||2015|
Vascular pathology of 20-month-old hypercholesterolemia mice in comparison to triple-transgenic and APPSwDI Alzheimer's disease mouse models.
71-5800 was used in immunohistochemistry to investigate hypercholesterolemia at various stages of Alzheimer's disease
|Hohsfield LA,Daschil N,Orädd G,Strömberg I,Humpel C||Molecular and cellular neurosciences (63:83)||2014|
D-Ala2GIP facilitated synaptic plasticity and reduces plaque load in aged wild type mice and in an Alzheimer's disease mouse model.
71-5800 was used in immunohistochemistry to test if D-Ala2GIP protects mice with advanced staged Alzheimer's disease.
|Faivre E,Hölscher C||Journal of Alzheimer's disease : JAD (35:267)||2013|
Tissue transglutaminase colocalizes with extracellular matrix proteins in cerebral amyloid angiopathy.
71-5800 was used in immunohistochemistry to determine the distribution pattern, cellular source, and activity of tissue transglutaminase in cerebral amyloid angiopathy, Alzheimer's disease, and hereditary cerebral hemorrhage with amyloidosis of the Dutch type cases.
|de Jager M,van der Wildt B,Schul E,Bol JG,van Duinen SG,Drukarch B,Wilhelmus MM||Neurobiology of aging (34:1159)||2013|
Evaluation of the effects of testosterone and luteinizing hormone on regulation of ?-amyloid in male 3xTg-AD mice.
71-5800 was used in immunohistochemistry to examine how changes in testosterone and luteinizing hormone contribute to Alzheimer's disease.
|Rosario ER,Carroll JC,Pike CJ||Brain research (1466:137)||2012|
An anti-diabetes agent protects the mouse brain from defective insulin signaling caused by Alzheimer's disease- associated Aß oligomers.
71-5800 was used in immunohistochemistry to investigate dysregulated insulin signaling in Alzheimer's disease and diabetes.
|Bomfim TR,Forny-Germano L,Sathler LB,Brito-Moreira J,Houzel JC,Decker H,Silverman MA,Kazi H,Melo HM,McClean PL,Holscher C,Arnold SE,Talbot K,Klein WL,Munoz DP,Ferreira ST,De Felice FG||The Journal of clinical investigation (122:1339)||2012|
Effects of aromatase inhibition versus gonadectomy on hippocampal complex amyloid pathology in triple transgenic mice.
71-5800 was used in immunohistochemistry to assess the effects of depleting both circulating and brain estrogen levels in a mouse model of Alzheimer's disease.
|Overk CR,Lu PY,Wang YT,Choi J,Shaw JW,Thatcher GR,Mufson EJ||Neurobiology of disease (45:479)||2012|
Astroglial connexin immunoreactivity is specifically altered at ß-amyloid plaques in ß-amyloid precursor protein/presenilin1 mice.
71-5800 was used in immunohistochemistry to determine the expression pattern of connexin43 and 30 in two murine models of Alzheimer disease
|Mei X,Ezan P,Giaume C,Koulakoff A||Neuroscience (171:92)||2010|
Selective estrogen receptor modulators differentially regulate Alzheimer-like changes in female 3xTg-AD mice.
71-5800 was used in immunohistochemistry to assess the use of selective estrogen receptor modulators using a murine model of Alzheimer's disease.
|Carroll JC,Pike CJ||Endocrinology (149:2607)||2008|
Progesterone and estrogen regulate Alzheimer-like neuropathology in female 3xTg-AD mice.
71-5800 was used in immunohistochemistry to examine the individual and combined effects of estrogen and progesterone on beta-amyloid accumulation, tau hyperphosphorylation, and hippocampal-dependent behavioral impairments
|Carroll JC,Rosario ER,Chang L,Stanczyk FZ,Oddo S,LaFerla FM,Pike CJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:13357)||2007|
Cholinergic degeneration is associated with increased plaque deposition and cognitive impairment in APPswe/PS1dE9 mice.
71-5800 was used in ELISA to study cholinergic dysfunction in a murine model of Alzheimer's disease.
|Laursen B,M?rk A,Plath N,Kristiansen U,Bastlund JF||Behavioural brain research (240:146)||2013|
|Not Applicable||Not Cited||
Patients with Alzheimer disease have lower levels of serum anti-amyloid peptide antibodies than healthy elderly individuals.
71-5800 was used in ELISA to determine the link between lower levels of serum anti-amyloid peptide antibodies and patients with Alzheimer disease when compared to healthy elderly individuals
|Weksler ME,Relkin N,Turkenich R,LaRusse S,Zhou L,Szabo P||Experimental gerontology (37:943)||2002|
The effect of ageing on neurogenesis and oxidative stress in the APP(swe)/PS1(deltaE9) mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - free floating to examine how ageing affects neurogenesis and oxidative stress in a mouse model of Alzheimer's disease.
|Hamilton A,Holscher C||Brain research (1449:83)||2012|
Androgens regulate the development of neuropathology in a triple transgenic mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - free floating to study the relationship between androgen depletion and Alzheimer's disease
|Rosario ER,Carroll JC,Oddo S,LaFerla FM,Pike CJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:13384)||2006|
Effects of NK-4 in a transgenic mouse model of Alzheimer's disease.
71-5800 was used in immunohistochemistry - paraffin section to report the effects of NK-4 on the toxicity of Aβ, on cognitive function, and Aβ concentration in a mouse model of Alzheimer's disease.
|Ohta H,Arai S,Akita K,Ohta T,Fukuda S||PloS one (7:null)||2012|
Abeta-related angiitis: primary angiitis of the central nervous system associated with cerebral amyloid angiopathy.
71-5800 was used in immunohistochemistry - paraffin section to describe patients with Abeta-related angiitis
|Scolding NJ,Joseph F,Kirby PA,Mazanti I,Gray F,Mikol J,Ellison D,Hilton DA,Williams TL,MacKenzie JM,Xuereb JH,Love S||Brain : a journal of neurology (128:500)||2005|
Amyloid-beta deposition in skeletal muscle of transgenic mice: possible model of inclusion body myopathy.
71-5800 was used in immunohistochemistry - paraffin section to characterize transgenic mice that overexpress the signal plus 99-amino acid carboxyl-terminal sequences of beta protein precursor
|Fukuchi K,Pham D,Hart M,Li L,Lindsey JR||The American journal of pathology (153:1687)||1998|
|Not Applicable||Not Cited||
Receptor-associated protein (RAP) plays a central role in modulating Abeta deposition in APP/PS1 transgenic mice.
71-5800 was used in western blot to discuss proteins that regulate the metabolism of the amyloid precursor protein and beta-amyloid peptides
|Xu G,Karch C,Li N,Lin N,Fromholt D,Gonzales V,Borchelt DR||PloS one (3:null)||2008|
|Not Applicable||Not Cited||
Gradual alteration of mitochondrial structure and function by beta-amyloids: importance of membrane viscosity changes, energy deprivation, reactive oxygen species production, and cytochrome c release.
71-5800 was used in western blot to test if gradual mitochondrial dysfunction contributes to the initiation and progression of sporadic Alzheimer's disease
|Aleardi AM,Benard G,Augereau O,Malgat M,Talbot JC,Mazat JP,Letellier T,Dachary-Prigent J,Solaini GC,Rossignol R||Journal of bioenergetics and biomembranes (37:207)||2005|
|Not Applicable||Not Cited||
Glutamate receptor subunit 3 is modified by site-specific limited proteolysis including cleavage by gamma-secretase.
71-5800 was used in western blot to investigate the processing and stability of the glutamate receptor
|Meyer EL,Strutz N,Gahring LC,Rogers SW||The Journal of biological chemistry (278:23786)||2003|
22R-Hydroxycholesterol protects neuronal cells from beta-amyloid-induced cytotoxicity by binding to beta-amyloid peptide.
71-5800 was used in western blot to elucidate the mechanism of action of 22R-hydroxycholesterol on Abeta-mediated toxicity.
|Yao ZX,Brown RC,Teper G,Greeson J,Papadopoulos V||Journal of neurochemistry (83:1110)||2002|
Rapid detection of protein aggregates in the brains of Alzheimer patients and transgenic mouse models of amyloidosis.
71-5800 was used in western blot to develop a novel method for the rapid detection of beta-amyloid aggregates in Alzheimer's patients and transgenic mouse models.
|Xu G,Gonzales V,Borchelt DR||Alzheimer disease and associated disorders (16:191)||2002|
Distinct binding sites in the structure of alpha 2-macroglobulin mediate the interaction with beta-amyloid peptide and growth factors.
71-5800 was used in western blot to study the mediation of the interaction with beta-amyloid peptide and growth factors due to distinct binding sites in the structure of alpha 2-macroglobulin
|Mettenburg JM,Webb DJ,Gonias SL||The Journal of biological chemistry (277:13338)||2002|
|Not Applicable||5 µg/ml||
Sporadic inclusion body myositis correlates with increased expression and cross-linking by transglutaminases 1 and 2.
71-5800 was used in immunohistochemistry and western blot to investigate the role of transglutaminases in sporadic inclusion body myositis
|Choi YC,Park GT,Kim TS,Sunwoo IN,Steinert PM,Kim SY||The Journal of biological chemistry (275:8703)||2000|
|Not Applicable||Not Cited||
Rodent A beta modulates the solubility and distribution of amyloid deposits in transgenic mice.
71-5800 was used in immunohistochemistry - paraffin section, immunoprecipitation, and western blot to explore the amyloidogenic potential of mouse A beta
|Jankowsky JL,Younkin LH,Gonzales V,Fadale DJ,Slunt HH,Lester HA,Younkin SG,Borchelt DR||The Journal of biological chemistry (282:22707)||2007|