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      View (44) other beta Amyloid antibodies

      Invitrogen

      beta Amyloid Polyclonal Antibody (CT695)

      Published Figures 40 Published Figures
      References Icon 93 References
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      Catalog # 51-2700

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      Product Details

      TESTED APPLICATIONS DILUTION
      ELISA (ELISA) 0.1-1.0 ug/ml
      Immunofluorescence (IF) 1-5 ug/ml
      Immunohistochemistry (IHC) 0.1-1.0 ug/ml
      Western Blot (WB) 0.2-2 ug/ml
      PUBLISHED APPLICATIONS
      Immunohistochemistry (IHC) See 23 publications below
      Immunohistochemistry (Frozen) (IHC (F)) See 7 publications below
      Immunohistochemistry - Free Floating (IHC (Free)) See 4 publications below
      Western Blot (WB) See 27 publications below
      Immunocytochemistry (ICC) See 4 publications below
      Immunofluorescence (IF) See 12 publications below
      Miscellaneous PubMed (MISC) See 6 publications below
      Immunoprecipitation (IP) See 7 publications below
      ELISA (ELISA) See 1 publications below
      Immunohistochemistry (Paraffin) (IHC (P)) See 1 publications below
      Immunomicroscopy (IM) See 1 publications below
      Species reactivity Human, Mouse, Pig, Rat
      Published species Hamster, Human, Mouse, Non-human primate, Pig, Rat, Tag
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Clone CT695
      Immunogen A 22 amino acid synthetic peptide derived from the C-terminus of the human beta-amyloid precursor protein (beta-APP). This sequence is 100% conserved in human, monkey, mouse, rat, guinea pig, and chicken beta-APP proteins.
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.25 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS, pH 7.4
      Contains 0.1% sodium azide
      Storage conditions -20°C
      RRID AB_2533902

      Product Specific Information

      This antibody can be used to specifically detect the beta-amyloid precursor protein. The antibody reacts with full-length (APP695, 751, 770) and N-terminal truncated forms of beta-APP. The antibody can also be used to detect the C-terminal membrane-anchored fragment of beta-APP that remains after alpha- or beta-secretase cleavage. This antibody does not detect the beta-APP product N-terminal to the gamma-secretase cleavage site.

      Reactivity with this antibody has been confirmed for human, mouse, pig, and rat. Based on sequence homology, reactivity with other species, including monkey, guinea pig, and chicken beta-APP, is highly likely. The CT695 antibody has demonstrated superiority in detecting axonal damage.

      This antibody has been tested in western blotting with FRTL-5 (rat thyroid) cells and rat brain lysates. For immunohistochemistry FFPE tissues will require epitope retrieval pretreatment.

      Background/Target Information

      Amyloid beta peptide (Abeta), 4 kDa, is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta-APP) in a two-step process (internal proteolysis). The first step involves cleavage of the extracellular, N-terminal domain of beta-APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. beta-APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta-APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. gamma-Secretase cleaves an intra-membrane site in the C-terminal domain of beta-APP, thus generating the Abeta peptide. gamma-Secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the C-terminus of abeta-APP and helps to modulate the production of the Abeta peptide. The major brain beta-APP isoforms (APP695, 751, 770) are integral membrane proteins with a single large N-terminal extracellular domain, a single transmembrane domain, and a small cytoplasmic C-terminal tail. In the early 1990s, it was discovered that beta-APP is a useful marker for axonal injury. beta-APP is carried by fast anterograde transport vesicles to distal sites in the axon with the beta-APP C-terminus residing in the cytoplasm and the N-terminus in the vesicle. Upon axonal injury, beta-APP will pool in areas of impaired transport. beta-APP has been used as a marker of axonal injury in many studies of different pathologies, including: head trauma, HTLV-I-associated myelopathy, multiple sclerosis, shaken baby syndrome, and encephalomyelitis among others.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (93)

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      Bioinformatics

      Protein Aliases: AAA; ABETA; ABPP; AD1; AG; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid A4; amyloid beta (A4) precursor protein; amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease); Amyloid beta A4 protein; Amyloid precursor protein; Amyloidogenic glycoprotein; Amyloidogenic glycoprotein AG; APP; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); Beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Peptidase nexin-II; PN-II; PN2; PreA4; protease nexin II; Protease nexin-II; testicular tissue protein Li 2

      Gene Aliases: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2

      UniProt ID: (Human) P05067, (Pig) P79307, (Rat) P08592, (Mouse) P12023

      Entrez Gene ID: (Human) 351, (Pig) 397663, (Rat) 54226, (Mouse) 11820

      Molecular Function: signaling molecule

      Function(s) Blue Arrow Icon
      response to yeast suckling behavior platelet degranulation mRNA polyadenylation regulation of translation protein phosphorylation cellular copper ion homeostasis endocytosis response to oxidative stress cell adhesion regulation of epidermal growth factor-activated receptor activity Notch signaling pathway axonogenesis mating behavior locomotory behavior axo-dendritic transport cholesterol metabolic process adult locomotory behavior visual learning cellular process response to lead ion negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle axon midline choice point recognition neuron remodeling dendrite development antibacterial humoral response antifungal humoral response extracellular matrix organization forebrain development neuron projection development ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth regulation of protein binding cellular protein metabolic process innate immune response negative regulation of neuron differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter collateral sprouting in absence of injury regulation of synapse structure or activity defense response to Gram-negative bacterium defense response to Gram-positive bacterium neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis cellular response to cAMP cellular response to norepinephrine stimulus amyloid fibril formation cellular response to nerve growth factor stimulus regulation of gene expression apoptotic process nervous system development negative regulation of peptidase activity protein homooligomerization
      Process(es) Blue Arrow Icon
      DNA binding
       serine-type endopeptidase inhibitor activity
       receptor binding
       protein binding
       heparin binding
       peptidase activator activity
       enzyme binding
       identical protein binding
       transition metal ion binding
       PTB domain binding
       growth factor receptor binding
       acetylcholine receptor binding
       peptidase inhibitor activity
       metal ion binding

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