|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunofluorescence (IF)||1-5 ug/ml|
|Immunohistochemistry (IHC)||0.1-1.0 ug/ml|
|Western Blot (WB)||0.2-2 ug/ml|
|Immunohistochemistry (IHC)||See 23 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 7 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 4 publications below|
|Western Blot (WB)||See 27 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunofluorescence (IF)||See 12 publications below|
|Miscellaneous PubMed (MISC)||See 6 publications below|
|Immunoprecipitation (IP)||See 7 publications below|
|ELISA (ELISA)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunomicroscopy (IM)||See 1 publications below|
|Species reactivity||Human, Mouse, Pig, Rat|
|Published species||Hamster, Human, Mouse, Non-human primate, Pig, Rat, Tag|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 22 amino acid synthetic peptide derived from the C-terminus of the human beta-amyloid precursor protein (beta-APP). This sequence is 100% conserved in human, monkey, mouse, rat, guinea pig, and chicken beta-APP proteins.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody can be used to specifically detect the beta-amyloid precursor protein. The antibody reacts with full-length (APP695, 751, 770) and N-terminal truncated forms of beta-APP. The antibody can also be used to detect the C-terminal membrane-anchored fragment of beta-APP that remains after alpha- or beta-secretase cleavage. This antibody does not detect the beta-APP product N-terminal to the gamma-secretase cleavage site.
Reactivity with this antibody has been confirmed for human, mouse, pig, and rat. Based on sequence homology, reactivity with other species, including monkey, guinea pig, and chicken beta-APP, is highly likely. The CT695 antibody has demonstrated superiority in detecting axonal damage.
This antibody has been tested in western blotting with FRTL-5 (rat thyroid) cells and rat brain lysates. For immunohistochemistry FFPE tissues will require epitope retrieval pretreatment.
Amyloid beta peptide (Abeta), 4 kDa, is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta-APP) in a two-step process (internal proteolysis). The first step involves cleavage of the extracellular, N-terminal domain of beta-APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. beta-APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta-APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. gamma-Secretase cleaves an intra-membrane site in the C-terminal domain of beta-APP, thus generating the Abeta peptide. gamma-Secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the C-terminus of abeta-APP and helps to modulate the production of the Abeta peptide. The major brain beta-APP isoforms (APP695, 751, 770) are integral membrane proteins with a single large N-terminal extracellular domain, a single transmembrane domain, and a small cytoplasmic C-terminal tail. In the early 1990s, it was discovered that beta-APP is a useful marker for axonal injury. beta-APP is carried by fast anterograde transport vesicles to distal sites in the axon with the beta-APP C-terminus residing in the cytoplasm and the N-terminus in the vesicle. Upon axonal injury, beta-APP will pool in areas of impaired transport. beta-APP has been used as a marker of axonal injury in many studies of different pathologies, including: head trauma, HTLV-I-associated myelopathy, multiple sclerosis, shaken baby syndrome, and encephalomyelitis among others.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAA; ABETA; ABPP; AD1; AG; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid A4; amyloid beta (A4) precursor protein; amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease); Amyloid beta A4 protein; Amyloid precursor protein; Amyloidogenic glycoprotein; Amyloidogenic glycoprotein AG; APP; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); Beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Peptidase nexin-II; PN-II; PN2; PreA4; protease nexin II; Protease nexin-II; testicular tissue protein Li 2
Gene Aliases: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2
Molecular Function: signaling molecule
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.