PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.
PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.
The PA1-730 immunizing peptide corresponds to amino acid residues 384-397 from human beta-arrestin2. This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: arrestin 2; arrestin 3; Arrestin beta-1; Arrestin beta-2; arrestin, beta 1; arrestin, beta 2; Beta-arrestin-1; Beta-arrestin-2; beta-arrestin2; Non-visual arrestin-2; Non-visual arrestin-3; S-Arrestin
Gene Aliases: ARB1; ARB2; ARR1; ARR2; ARRB1; ARRB2; BARR2; BARRES
Molecular Function: enzyme modulator