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Invitrogen
beta COP Polyclonal Antibody
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FIGURE: 1 / 20
beta COP Antibody (PA1-061) in ICC/IF
Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta COP Rabbit Polyclonal Antibody (Product # PA1-061) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA1-061
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
C. elegans,
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human and rat tissues.
PA1-061 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate. Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells with PA1-061 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.
The PA1-061 immunogen is a synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. This sequence is completely conserved in human beta-COP.
Target Information
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta coat protein; Beta-coat protein; Beta-COP; coatomer protein complex, subunit beta 1; Coatomer subunit beta; DKFZp761K102; FLJ10341; FLJ46444; FLJ57957; receptor for activated C-kinase-2
Gene Aliases: 2610019B04Rik; COPB; COPB1; MSTP026; Rack2
UniProt ID: (Human) P53618, (Mouse) Q9JIF7, (Rat) P23514
Entrez Gene ID: (Human) 1315, (Mouse) 70349, (Rat) 114023
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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