Catalog # AHO0462
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 1 publications below|
|Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the C-terminus of the beta-catenin protein|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
This antibody reacts with the C-terminal portion of beta-Catenin.
For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls are MCF-7 cells and breast carcinoma tissue.
The catenins (alpha, beta and gamma) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between alpha-actinin and alpha-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta-catenin; catenin beta-1
Gene Aliases: armadillo; Bfc; Catnb; CTNNB; Mesc; MRD19; OK/SW-cl.35
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