Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin Rabbit Polyclonal Antibody (PA141331) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Dog, Chicken, Cat, Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(417) L V S E Y Q Q Y Q D A T A D E Q G E(435) of human b-Tubulin.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||1 - 2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Suggested positive control: antigen standard for TUBB2A (transient overexpression lysate), human brain protein.
Alpha and beta Tubulin are the most common members of the tubulin family of proteins. Dimers of alpha and beta tubulin assemble to form microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reg3¿ Overexpression Protects Pancreatic Beta-Cells From Cytokine-Induced Damage and Improves Islet Transplant Outcome.
PA1-41331 was used in western blot to determine if Reg3alpha improves islet engraftment
|Ding Y,Xu Y,Shuai X,Shi X,Chen X,Huang W,Liu Y,Liang X,Zhang Z,Su D||Molecular medicine (Cambridge, Mass.) (null:null)||2014|
The neurotensin-HIF-1¿-VEGF¿ axis orchestrates hypoxia, colonic inflammation, and intestinal angiogenesis.
PA1-41331 was used in western blot to study the role of neurotensin and its receptor in both colitis and inflammatory bowel disease
|Bakirtzi K,West G,Fiocchi C,Law IK,Iliopoulos D,Pothoulakis C||The American journal of pathology (184:3405)||2014|