Invitrogen

beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 800 4X PEG

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beta Tubulin Loading Control Antibody in Western Blot (WB) — Western blot analysis of beta-Tubulin was performed by loading 75 µg of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK Blocking Buffer (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated beta-Tubulin monoclonal antibody (Product # MA5-16308-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform, and washed in TBS-0.1% Tween-20. Fluorescent detection was performed using a near IR imaging system.

Product Details

MA5-16308-D800

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	BT7R
Immunogen	KLH conjugated peptide of the N-terminus of Beta-Tubulin.
Conjugate	DyLight™ 800 4X PEG Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647 Biotin DyLight 488 DyLight 550 DyLight 650 DyLight 680 HRP Unconjugated
Excitation/Emission Max	789/794 nm View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	MES with proprietary stabilizer
Contains	no preservative
Storage conditions	4° C, do not freeze
RRID	AB_2537826

Product Specific Information

MA5-16308-D800 detects bet-Tubulin from various species. MA5-16308-D800 has been successfully used for fluorescent detection of beta-Tubulin in Western Blotting.

DyLight 800 4X PEG has an excitation/emission of 783/797 nm.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; betaIII-tubulin; class IIa beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2A class IIa; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta, 5

Gene Aliases: 2310057H16Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA631M21.2; BB220206; beta-4; beta-5; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; RGD1305887; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8

UniProt ID: (Human) P07437, (Human) Q9H4B7, (Human) Q13885, (Human) Q13509, (Human) P04350, (Human) Q9BUF5, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Rat) P69897, (Mouse) P99024, (Mouse) Q922F4