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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
BT7R |
Immunogen |
KLH conjugated peptide of the N-terminus of Beta-Tubulin. |
Conjugate |
DyLight™ 800 4X PEG
|
Excitation/Emission Max |
789/794 nm View spectra |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
MES with proprietary stabilizer |
Contains |
no preservative |
Storage conditions |
4° C, do not freeze |
RRID |
AB_2537826 |
MA5-16308-D800 detects bet-Tubulin from various species. MA5-16308-D800 has been successfully used for fluorescent detection of beta-Tubulin in Western Blotting.
DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; betaIII-tubulin; class IIa beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2A class IIa; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta, 5
Gene Aliases: 2310057H16Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA631M21.2; BB220206; beta-4; beta-5; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; RGD1305887; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8
UniProt ID: (Human) P07437, (Human) Q9H4B7, (Human) Q13885, (Human) Q13509, (Human) P04350, (Human) Q9BUF5, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Rat) P69897, (Mouse) P99024, (Mouse) Q922F4
Entrez Gene ID: (Human) 203068, (Human) 81027, (Human) 7280, (Human) 10381, (Human) 10382, (Human) 84617, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Rat) 29214, (Mouse) 22154, (Mouse) 67951, (Rat) 307351
Molecular Function:
tubulin
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