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Invitrogen
c-Kit Monoclonal Antibody (2B8), Alexa Fluor™ 647
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FIGURE: 1 / 1
c-Kit Antibody (RM6221) in IHC
Cell death & disease 2016 -
Figure 3 c-Kit + cardiac cell distribution is not affected by c-Kit TgD814Y mutation in vivo . ( a ) Immunofluorescence on heart sections prepared from 1 dpp, 10 dpp and adult c-Kit TgD814Y pKit EGFP mice showing intramyocardial (1 dpp and Adult) and peri-vasal (10 dpp) c-Kit + cells localization. Insets show c-Kit (red) and EGFP (green) co-expression. Scale bars 30 mu M. ( b ) Count of EGFP + cells in c-Ki... View More
Product Details
RM6221
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
2B8
Immunogen
IL-3-dependent mast cells derived from normal bone marrow.
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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