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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
Assay-dependent | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
Assay-dependent | View 2 publications 2 publications |
Flow Cytometry (Flow) |
0.2 µg/1x10^6 cells | - |
ELISA (ELISA) |
Assay-dependent | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Neutralization (Neu) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rat / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
ACK4 |
Immunogen |
IL-3 dependent mast cells derived from WB- +/+ mice |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2249527 |
c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit
Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W
UniProt ID: (Mouse) P05532
Entrez Gene ID: (Mouse) 16590
Molecular Function:
transmembrane signal receptor
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