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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
Assay-dependent | - |
Immunohistochemistry (Frozen) (IHC (F)) |
Assay-dependent | - |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | View 3 publications 3 publications |
Immunohistochemistry (IHC) |
- | View 4 publications 4 publications |
Western Blot (WB) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse, Pig |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
K45 |
Immunogen |
Recombinant human extracellular c-kit fragment |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4, with 0.2% BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_10990055 |
MA5-12944 targets CD117 in IF/ICC and IHC (P, F) applications and shows reactivity with Human samples.
The MA5-12944 immunogen is recombinant human extracellular c-kit fragment.
c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR
UniProt ID: (Human) P10721
Entrez Gene ID: (Human) 3815
Molecular Function:
transmembrane signal receptor
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