|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||IL-3-dependent mast cells derived from normal bone marrow.|
|Conjugate||Alexa Fluor® 647|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury.
RM6221 was used in flow cytometry use transgenic mice to study how endogenous c-Kit receptor activation affects cardiac cell homeostasis and repair
|Di Siena S,Gimmelli R,Nori SL,Barbagallo F,Campolo F,Dolci S,Rossi P,Venneri MA,Giannetta E,Gianfrilli D,Feigenbaum L,Lenzi A,Naro F,Cianflone E,Mancuso T,Torella D,Isidori AM,Pellegrini M||Cell death and disease (7:null)||2016|
Lymphotoxin-independent expression of TNF-related activation-induced cytokine by stromal cells in cryptopatches, isolated lymphoid follicles, and Peyer's patches.
RM6221 was used in immunohistochemistry - frozen section to investigate how TRANCE on stromal cells contributes to the differentiation and maintenance of organized lymphoid aggregates in the small intestine.
|Taylor RT,Patel SR,Lin E,Butler BR,Lake JG,Newberry RD,Williams IR||Journal of immunology (Baltimore, Md. : 1950) (178:5659)||2007|