Invitrogen

c-Raf Polyclonal Antibody

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c-Raf Polyclonal Antibody

Product Details

PA5-95464

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Raf1 recombinant protein (Position: Y364-Q451).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807266

Product Specific Information

Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat kidney tissue, mouse kidney tissue, human MCF-7 whole cell, human 22RV1 whole cell, human Hela whole cell. Flow: HeLa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-RAF; C-Raf proto-oncogene, serine/threonine kinase; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le

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Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf

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UniProt ID: (Human) P04049, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Human) 5894, (Mouse) 110157, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding metal ion binding protein heterodimerization activity nucleotide binding receptor signaling protein activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity response to hypoxia stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process signal transduction activation of adenylate cyclase activity heart development cell proliferation negative regulation of cell proliferation cell differentiation platelet activation thyroid gland development negative regulation of protein complex assembly positive regulation of peptidyl-serine phosphorylation ion transmembrane transport somatic stem cell population maintenance regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch wound healing regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway thymus development face development death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell motility cellular glucose homeostasis positive regulation of protein phosphorylation phosphorylation intracellular signal transduction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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