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Invitrogen
cGAS Polyclonal Antibody
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FIGURE: 1 / 2
cGAS Antibody (PA5-76367) in WB
Western blot was performed using Anti-C6orf150 Polyclonal Antibody (Product # PA5-76367) and a 60 kDa band corresponding to Cyclic GMP-AMP synthase along with an uncharacterized band (*) at ~130 kDa were observed across high expressing HeLa, PANC-1 compared to low expressing BeWo, IMR-32 and SH-SY5Y cells. Whole cell extracts (40 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), BeWo (Lane 3), IMR-32 (Lane 4), SH-SY5Y (Lane 5), Rat Heart (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved prot... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-76367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide of human C6orf150.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2'3'-cGAMP synthase; C6orf150; C6orf150; cGAS is a cytosolic DNA sensor that induces interferons by producing the second messenger cGAMP; cGAMP synthase; DANGER family member 4; h-cGAS; Mab-21 domain containing 1; mab-21 domain-containing protein 1; MB21D1; protein MB21D1; unnamed protein product
Gene Aliases: C6orf150; D4; h-cGAS; MB21D1
UniProt ID: (Human) Q8N884
Entrez Gene ID: (Human) 115004, (Rat) 682147
DNA binding
chromatin binding
double-stranded DNA binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
nucleosome binding
identical protein binding
protein homodimerization activity
cyclic-GMP-AMP synthase activity
molecular condensate scaffold activity
poly-ADP-D-ribose modification-dependent protein binding
activation of innate immune response
pattern recognition receptor signaling pathway
positive regulation of defense response to virus by host
regulation of immunoglobulin production
cytoplasmic pattern recognition receptor signaling pathway
cellular response to DNA damage stimulus
determination of adult lifespan
regulation of type I interferon production
positive regulation of type I interferon production
paracrine signaling
negative regulation of DNA repair
regulation of immune response
regulation of T cell activation
defense response to virus
cellular response to exogenous dsRNA
antiviral innate immune response
cGAS/STING signaling pathway
negative regulation of double-strand break repair via homologous recombination
positive regulation of cellular senescence
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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