Proteintech

cIAP1 Polyclonal Antibody

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cIAP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 10022-1-AP) (cIAP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

cIAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 10022-1-AP ( cIAP1 Antibody) at dilution of 1:200 (under 40x lens).

cIAP1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

10022-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

cIAP1 Fusion Protein Ag15203 (1-350 aa encoded by BC016174)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This BIRC2 antibody (10022-1-AP) can bind both full length (70 kDa) and cleaved form (60 kDa) of the protein.

Immunogen sequence: MHKTASQRL FPGPSYQNIK SIMEDSTILS DWTNSNKQKM KYDFSCELYR MSTYSTFPAG VPVSERSLAR AGFYYTGVND KVKCFCCGLM LDNWKLGDSP IQKHKQLYPS CSFIQNLVSA SLGSTSKNTS PMRNSFAHSL SPTLEHSSLF SGSYSSLSPN PLNSRAVEDI SSSRTNPYSY AMSTEEARFL TYHMWPLTFL SPSELARAGF YYIGPGDRVA CFACGGKLSN WEPKDDAMSE HRRHFPNCPF LENSLETLRF SISNLSMQTH AARMRTFMYW PSSVPVQPEQ LASAGFYYVG RNDDVKCFCC DGGLRCWESG DDPWVEHAKW FPRCEFLIRM KGQEFVDEIQ G (1-350 aa encoded by BC016174)

Target Information

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Cell death & disease

Li M,Wei Y,Liu Y,Wei J,Zhou X,Duan Y,Chen S,Xue C,Zhan Y,Zheng L,Deng H,Tang F,Fan S,Xiong W,Li G,Tan M,Zhou M

Tue Feb 14 00:00:00 EST 2023

TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein.

Journal of experimental & clinical cancer research : CR

Yang X,Zhang Y,Xue Z,Hu Y,Zhou W,Xue Z,Liu X,Liu G,Li W,Liu X,Li X,Han M,Wang J

Tue Dec 06 00:00:00 EST 2022

The Antigastric Cancer Effect of Triptolide is Associated With H19/NF-κB/FLIP Axis.

Frontiers in pharmacology

Yuan W,Huang J,Hou S,Li H,Bie L,Chen B,Li G,Zhou Y,Chen X

Sat Sep 17 00:00:00 EDT 2022

Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.

Molecular cell

Du M,Ea CK,Fang Y,Chen ZJ

Thu Jul 07 00:00:00 EDT 2022

TRAF3 in T Cells Restrains Negative Regulators of LAT to Promote TCR/CD28 Signaling.

Journal of immunology (Baltimore, Md. : 1950)

Arkee T,Hostager BS,Houtman JCD,Bishop GA

Thu Jul 01 00:00:00 EDT 2021

Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wang L,Du H,Chen P

Sun Nov 01 00:00:00 EDT 2020

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- B and NF- B-mediated cellular FLICE-inhibitory protein.

Acta pharmaceutica Sinica. B

Yuan Z,Yuan Z,Hasnat M,Zhang H,Liang P,Sun L,Jiang Z,Zhang L

Fri May 01 00:00:00 EDT 2020

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Developmental cell

Zachari M,Gudmundsson SR,Li Z,Manifava M,Cugliandolo F,Shah R,Smith M,Stronge J,Karanasios E,Piunti C,Kishi-Itakura C,Vihinen H,Jokitalo E,Guan JL,Buss F,Smith AM,Walker SA,Eskelinen EL,Ktistakis NT

Mon Sep 09 00:00:00 EDT 2019

Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.

Cancer medicine

Chen SM,Lin TK,Tseng YY,Tu CH,Lui TN,Huang SF,Hsieh LL,Li YY

Wed Aug 01 00:00:00 EDT 2018

Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.

Cancer medicine

Chen SM,Lin TK,Tseng YY,Tu CH,Lui TN,Huang SF,Hsieh LL,Li YY

Wed Aug 01 00:00:00 EDT 2018

The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.

Oncotarget

Liu TH,Zheng F,Cai MY,Guo L,Lin HX,Chen JW,Liao YJ,Kung HF,Zeng YX,Xie D

Tue May 03 00:00:00 EDT 2016

The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.

Oncotarget

Liu TH,Zheng F,Cai MY,Guo L,Lin HX,Chen JW,Liao YJ,Kung HF,Zeng YX,Xie D

Tue May 03 00:00:00 EDT 2016

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.

Biology of reproduction

Nie D,Zhang D,Dai J,Zhang M,Zhao X,Xu W,Chen Z,Wang L,Wang Z,Qiao Z

Mon Feb 01 00:00:00 EST 2016

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.

Biology of reproduction

Nie D,Zhang D,Dai J,Zhang M,Zhao X,Xu W,Chen Z,Wang L,Wang Z,Qiao Z

Mon Feb 01 00:00:00 EST 2016

Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.

Biochemical and biophysical research communications

Wu P,Zhu X,Jin W,Hao S,Liu Q,Zhang L

Fri May 01 00:00:00 EDT 2015

Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.

Biochemical and biophysical research communications

Wu P,Zhu X,Jin W,Hao S,Liu Q,Zhang L

Fri May 01 00:00:00 EDT 2015

Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.

Journal of immunology (Baltimore, Md. : 1950)

Peters AL,Bishop GA

Wed Dec 01 00:00:00 EST 2010

Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.

Journal of immunology (Baltimore, Md. : 1950)

Peters AL,Bishop GA

Wed Dec 01 00:00:00 EST 2010

A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.

Molecular cell

Hinz M,Stilmann M,Arslan SÇ,Khanna KK,Dittmar G,Scheidereit C

Fri Oct 08 00:00:00 EDT 2010

A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.

Molecular cell

Hinz M,Stilmann M,Arslan SÇ,Khanna KK,Dittmar G,Scheidereit C

Fri Oct 08 00:00:00 EDT 2010

HOIL-1L interacting protein (HOIP) as an NF-kappaB regulating component of the CD40 signaling complex.

PloS one

Hostager BS,Fox DK,Whitten D,Wilkerson CG,Eipper BA,Francone VP,Rothman PB,Colgan JD

Wed Jun 30 00:00:00 EDT 2010

HOIL-1L interacting protein (HOIP) as an NF-kappaB regulating component of the CD40 signaling complex.

PloS one

Hostager BS,Fox DK,Whitten D,Wilkerson CG,Eipper BA,Francone VP,Rothman PB,Colgan JD

Wed Jun 30 00:00:00 EDT 2010

Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells.

Leukemia research

Akao Y,Nakagawa Y,Iio A,Naoe T

Sun Nov 01 00:00:00 EDT 2009

Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells.

Leukemia research

Akao Y,Nakagawa Y,Iio A,Naoe T

Sun Nov 01 00:00:00 EDT 2009

c-IAP1 and c-IAP2 are critical mediators of tumor necrosis factor alpha (TNFalpha)-induced NF-kappaB activation.

The Journal of biological chemistry

Varfolomeev E,Goncharov T,Fedorova AV,Dynek JN,Zobel K,Deshayes K,Fairbrother WJ,Vucic D

Fri Sep 05 00:00:00 EDT 2008

c-IAP1 and c-IAP2 are critical mediators of tumor necrosis factor alpha (TNFalpha)-induced NF-kappaB activation.

The Journal of biological chemistry

Varfolomeev E,Goncharov T,Fedorova AV,Dynek JN,Zobel K,Deshayes K,Fairbrother WJ,Vucic D

Fri Sep 05 00:00:00 EDT 2008

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Nature genetics

Santoro MM,Samuel T,Mitchell T,Reed JC,Stainier DY

Thu Nov 01 00:00:00 EDT 2007

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Nature genetics

Santoro MM,Samuel T,Mitchell T,Reed JC,Stainier DY

Thu Nov 01 00:00:00 EDT 2007

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Nature genetics

Santoro MM,Samuel T,Mitchell T,Reed JC,Stainier DY

Thu Nov 01 00:00:00 EDT 2007

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Nature genetics

Santoro MM,Samuel T,Mitchell T,Reed JC,Stainier DY

Thu Nov 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: apoptosis inhibitor 1; apoptosis inhibitor 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing 3; Baculoviral IAP repeat-containing protein 2; C-IAP1; Cellular inhibitor of apoptosis 1; hIAP-2; IAP homolog B; IAP-2; Inhibitor of apoptosis protein 2; mIAP-2; mIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; TNFR2-TRAF-signaling complex protein 2

Gene Aliases: API1; Api2; AW146227; BIRC2; Birc3; c-IAP1; cIAP1; cIAP2; Hiap-2; HIAP1; HIAP2; IAP1; IAP2; mcIAP1; MIAP1; MIAP2; MIHB; MIHC; RNF48

UniProt ID: (Human) Q13490, (Mouse) Q62210

Entrez Gene ID: (Human) 329, (Mouse) 11797

Molecular Function: protease inhibitor