Proteintech

calreticulin Polyclonal Antibody

Product Image Gallery

calreticulin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 10292-1-AP (calreticulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

calreticulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 10292-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).

calreticulin Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Western Blot (WB)

1:500-1:1000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

calreticulin Fusion Protein Ag0325

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)

Gene Aliases: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA

UniProt ID: (Human) P27797, (Mouse) P14211, (Rat) P18418

Entrez Gene ID: (Human) 811, (Mouse) 12317, (Rat) 64202

Molecular Function: calcium-binding protein