Immunofluorescent analysis of cdc42 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a cdc42 polyclonal antibody (Product # PA1-092) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C-Terminal truncated human recombinant protein|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-092 has been successfully used in Western blot, IHC (P) and immunofluorescence applications with human, mouse, rat and monkey samples.
Cell division control protein 42 (Cdc42) is a plasma membrane-associated small GTPase of the Rho-family which cycles between an active GTP-bound and an inactive GDP-bound state. In its active state Cdc42 binds to effector proteins to regulate cellular responses such as cell morphology, migration, endocytosis and cell cycle progression. Cdc42 has also been shown to play a role in epithelial cell polarization processes and the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The localisation of the apical Par/Cdc42 polarity module is specifically affected in microvillus inclusion disease.
PA1-092 was used in immunohistochemistry - paraffin section to determine the apical polarity determinants in five patients with microvillus inclusion disease
|Michaux G,Massey-Harroche D,Nicolle O,Rabant M,Brousse N,Goulet O,Le Bivic A,Ruemmele FM||Biology of the cell (108:19)||2016|