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Agrisera
eEF1b/Elongation factor 1-beta Polyclonal Antibody
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FIGURE: 1 / 1
eEF1b/Elongation factor 1-beta Antibody (AS07-265) in WB
10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant e EF 1 b (3), 4 ng of recombinant e EF 1 b (4), 8 ng of recombinant e EF 1 b (5) were separated on 15% SDS-PAGE and blotted 1 h to nitrocellulose (Bio-Rad). Blots were incubated in the primary antibody at a dilution of 1:1,000 for 1 h at room temperature with agitation and... View More
Product Details
AS07-265
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Zea mays
Target Information
EF-1 (elongation factor-1) is a multi-protein complex that is responsible forthe delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complexis composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a225 amino acid member of the EF-1 β/EF-1 δ protein family and contains oneGST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange ofGDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5' UTR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EEF1B; EEF1B1; EF1B
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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