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eIF4E2 Antibody (PA5-34697) in WB
Western Blot using eIF4E2 Polyclonal Antibody (Product # PA5-34697). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E2 Polyclonal Antibody (Product # PA5-34697) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-34697
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 242 of EIF4E2 (Uniprot ID#O60573)
Conjugate
Form
Liquid
Concentration
0.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552049
Product Specific Information
Recommended positive controls: Target recombinant protein.
Predicted reactivity: Mouse (97%), Zebrafish (91%), Dog (98%), Pig (98%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4E type 2; eIF4E type 2; eIF4E-2; eIF4E-like cap-binding protein; eIF4E-like protein 4E-LP; eukar; Eukaryotic translation initiation factor 4E homologous protein; eukaryotic translation initiation factor 4E like 3; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; h4EHP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 2; mRNA cap-binding protein type 3
Gene Aliases: 2700069E09Rik; 4E-LP; 4EHP; AI036339; AV129531; D0H0S6743E; EIF4E2; EIF4EL3; IF4e
UniProt ID: (Human) O60573, (Mouse) Q8BMB3
Entrez Gene ID: (Human) 9470, (Mouse) 26987
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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