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Agrisera
mNeonGreen/ Fluorescent Protein Polyclonal Antibody
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FIGURE: 1 / 4
mNeonGreen/ Fluorescent Protein Antibody (AS21-4525) in ICC/IF
HEK 293 cells were transfected with the indicated plasmid (SARS-Co V-2 N Myc tagged, PMID: 34,799,561, TBEV NS 3-HA, PMID: 29,321,318, Rab 5 m Cherry, Add gene: 4,920, GFP-HIS) using gene juice transfection reagent (EMD Millipore) according to the manufacturer’s instructions. After 24 hours of transfection, cells were fixed in 4% formaldehyde and permeabilized in PBS containing 0.5% Triton X-100 and 20 m M ... View More
Product Details
AS21-4525
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from synthetic peptide from common lancelet Branchiostoma lanceolatum.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
Target Information
mNeonGreen is derived from the lancelet Branchiostoma lanceolatum multimeric yellow fluorescence protein. Hence, mNeonGreen is evolutionarily distant from jellyfish-derived fluorescent proteins. At sequence level, mNeonGreen shares just 20-25% sequence identity with common GFP derivatives. Due to the low sequence similarity to mNeonGreen, it is expected that in general affinity tools for GFP variants, i.e. antibodies, should not bind to mNeonGreen and vice versa. mNeonGreen consists of 237 amino acids, which translates into 26.6 kDa molecular weight. This size, but not sequence, is very similar to EGFP that comprises 239 amino acids and is 26.9 kDa. Furthermore, mNeonGreen has an excitation maximum at 506 nm and an emission maximum at 517 nm (Shaner et al., 2013). mNeonGreen is compatible with most GFP filter sets. Apparently, it is 3 times brighter than GFP when using GFP filters; optimization of filters may increase its brightness further. In addition, mNeonGreen seems to be more stable and less sensitive to laser induced bleaching than EGFP. Therefore, mNeonGreen is particular suitable for confocal and super resolution microscopy, especially when fusion proteins are investigated, which are expressed at low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mNeonGreen; mNG
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