Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
nNOS Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 16
nNOS Antibody (PA3-032A) in ICC/IF
Immunofluorescence analysis of nNOS was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with nNOS Rabbit Polyclonal Antibody (Product # PA3-032A) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
PA3-032A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rabbit,
Rat
Published species
C. elegans,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.
The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1
UniProt ID: (Human) P29475, (Rabbit) O19132, (Rat) P29476, (Mouse) Q9Z0J4
Entrez Gene ID: (Human) 4842, (Rabbit) 100009243, (Rat) 24598, (Mouse) 18125
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
sodium channel regulator activity
enzyme binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
ion channel binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
NADPH-hemoprotein reductase activity
amino acid binding
ATPase binding
oxidoreductase
protein modifying enzyme
response to hypoxia
regulation of sodium ion transport
arginine catabolic process
nitric oxide biosynthetic process
striated muscle contraction
nitric oxide mediated signal transduction
myoblast fusion
female pregnancy
aging
negative regulation of cell proliferation
response to heat
response to lead ion
negative regulation of calcium ion transport into cytosol
response to activity
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to lipopolysaccharide
response to vitamin E
multicellular organismal response to stress
positive regulation of histone acetylation
response to nicotine
neurotransmitter biosynthetic process
exogenous drug catabolic process
negative regulation of apoptotic process
negative regulation of potassium ion transport
response to peptide hormone
response to estrogen
cell redox homeostasis
response to ethanol
negative regulation of blood pressure
negative regulation of heart contraction
positive regulation of transcription, DNA-templated
negative regulation of vasoconstriction
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
behavioral response to cocaine
regulation of neurogenesis
negative regulation of hydrolase activity
negative regulation of cytosolic calcium ion concentration
negative regulation of serotonin uptake
negative regulation of calcium ion transport
regulation of sensory perception of pain
oxidation-reduction process
regulation of cardiac muscle contraction
regulation of ryanodine-sensitive calcium-release channel activity
cellular response to mechanical stimulus
cellular response to growth factor stimulus
response to nitric oxide
positive regulation of the force of heart contraction
positive regulation of long-term synaptic potentiation
negative regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
regulation of cardiac conduction
muscle contraction
regulation of heart contraction
response to organonitrogen compound
response to organic cyclic compound
response to nutrient levels
negative regulation of peptidyl-serine phosphorylation
cellular response to epinephrine stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support