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Invitrogen

nNOS Polyclonal Antibody

13 Published Figures
21 References
View all (32) nNOS antibodies

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nNOS Polyclonal Antibody

Product Details

PA3-032A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 6 publications 6 publications

Immunohistochemistry (IHC)

-
View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400
View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:800
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250
View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent
View 2 publications 2 publications
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rabbit, Rat

Published species

Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2236017

Product Specific Information

PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.

The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bNOS; bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; nNOS mu; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product

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Gene Aliases: 2310005C01Rik; bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I

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UniProt ID: (Human) P29475, (Rabbit) O19132, (Mouse) Q9Z0J4, (Rat) P29476

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Entrez Gene ID: (Human) 4842, (Rabbit) 100009243, (Mouse) 18125, (Rat) 24598

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Function(s)
nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding zinc ion binding enzyme binding identical protein binding ATPase binding phosphoprotein binding RNA polymerase II sequence-specific DNA binding transcription factor binding NADPH binding oxidoreductase protein modifying enzyme
Process(es)
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction cell communication myoblast fusion blood circulation response to heat response to hormone negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport signaling response to lipopolysaccharide multicellular organismal response to stress nitric oxide-cGMP-mediated signaling pathway xenobiotic catabolic process vasodilation negative regulation of potassium ion transport negative regulation of ion transport positive regulation of neuron apoptotic process cell redox homeostasis negative regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of cardiac muscle contraction regulation of postsynaptic membrane potential cellular response to growth factor stimulus positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential regulation of the force of heart contraction system process negative regulation of cell proliferation peptidyl-cysteine S-nitrosylation negative regulation of iron ion transmembrane transport negative regulation of apoptotic process response to peptide hormone negative regulation of neuron apoptotic process response to estrogen establishment of protein localization positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of vasoconstriction negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of hepatic stellate cell contraction cellular response to mechanical stimulus cellular response to epinephrine stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of long-term synaptic potentiation female pregnancy response to mechanical stimulus response to lead ion response to activity response to nutrient levels response to vitamin E response to vitamin B3 response to nicotine response to decreased oxygen levels regulation of apoptotic process response to ethanol nitric oxide metabolic process response to nitric oxide
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