Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta or hydatid mole.
p57/Kip 2 (or CDKN 1C) is a potent tight binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57/Kip 2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith Wiedemann syndrome (BWS, a cancer syndrome) making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin like growth factor II (IGF II) and H19, both of which seem to be implicated in adrenal neoplasms.
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Protein Aliases: BWCR; BWS; Cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C (p57, Kip2); Cyclin-dependent kinase inhibitor p57; IMAGE; KIP2; p57; p57Kip2; WBS
Gene Aliases: BWCR; BWS; CDKN1C; KIP2; p57; p57Kip2; WBS
UniProt ID: (Human) P49918
Entrez Gene ID: (Human) 1028