Transfection Protocols
Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell specific protocols for further optimization.
Transfection protocols
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| Cell type | Transfected nucleic acid | Transfection reagent |
|---|---|---|
| A549 human lung epithelial carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| A549 human lung epithelial carcinoma cells | plasmid DNA | Lipofectamine LTX |
| ACHN human renal adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| C6 rat glioma cells | plasmid DNA | Lipofectamine LTX |
| Caco-2 human adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| CHO-K1 cells | plasmid DNA | Lipofectamine LTX |
| COS-7 monkey kidney fibroblasts | plasmid DNA | Lipofectamine LTX |
| DU 145 human prostate epithelial carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| eukaryotic cells | DNA, RNA | DMRIE-C |
| eukaryotic cells | plasmid DNA | FreeStyle MAX |
| eukaryotic cells | DNA | Lipofectamine |
| eukaryotic cells | DNA, RNA | Lipofectamine 2000 |
| eukaryotic cells | DNA | Lipofectamine 2000 CD |
| eukaryotic cells | DNA | Lipofectamine LTX and PLUS |
| eukaryotic cells | siRNA, oligos | Oligofectamine |
| eukaryotic cells | pre-transfection DNA complexes | PLUS pre-transfection enhancer |
| FreeStyle 293-F cells | plasmid DNA | 293fectin |
| GripTite 293 MSR HECs | plasmid DNA | Lipofectamine LTX |
| HCT 116 human colorectal carcinoma cells | plasmid DNA | Lipofectamine LTX |
| HEK293 cells | plasmid DNA | Lipofectamine LTX |
| HEK293 suspension cells | plasmid DNA | 293fectin |
| HeLa S3 human cervical adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| Hep 3B2.1-7 human hepatocellular carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| Hep G2 human hepatocellular carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| Hep G2 human hepatocellular carcinoma cells | plasmid DNA | Lipofectamine LTX |
| Hepa 1-6 mouse hepatoma cells | plasmid DNA | Lipofectamine 3000 |
| HL-60 human lymphoblast cells | plasmid DNA | Lipofectamine LTX |
| Hs 578T human mammary epithelial carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| HT 29 human colorectal adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| HT-1080 human fibrosarcoma cells | plasmid DNA | Lipofectamine LTX |
| HUVEC | plasmid DNA | Lipofectamine LTX |
| HUVEC | siRNA, Stealth RNAi | RNAiMAX |
| IMR-90 human embryonic lung cells | plasmid DNA | Lipofectamine LTX |
| Jurkat human T cell leukemia cells | plasmid DNA | Lipofectamine LTX |
| K-562 human myelogenous leukemia cells | plasmid DNA | Lipofectamine LTX |
| LNCaP human prostate epithelial carcinoma cells | plasmid DNA | Lipofectamine 3000 |
| LNCaP human prostate epithelial carcinoma cells | plasmid DNA | Lipofectamine LTX |
| mammalian cells | dsRNA oligo (BLOCK-iT fluorescent oligo) | cationic lipid |
| mammalian cells | siRNA, Stealth RNAi + plasmid DNA (co-transfection) | Lipofectamine 2000 |
| mammalian cells | siRNA, Stealth RNAi | Lipofectamine 2000 |
| mammalian cells | DNA, RNA, oligos | Lipofectin |
| mammalian cells | siRNA, Stealth RNAi | RNAiMAX |
| mammalian cells, reverse transfection | siRNA, Stealth RNAi | Lipofectamine 2000 |
| mammalian cells, reverse transfection | siRNA, Stealth RNAi | RNAiMAX |
| MCF 10A human mammary epithelial cells | plasmid DNA | Lipofectamine 3000 |
| MCF7 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine 3000 |
| MCF7 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| MDA-MB-231 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine 3000 |
| MDA-MB-231 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| MDA-MB-435 cells | plasmid DNA | Lipofectamine LTX |
| MDA-MB-453 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine 3000 |
| MDA-MB-468 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine 3000 |
| MOLT-4 human T cell lymphoblast cells | plasmid DNA | Lipofectamine LTX |
| NCI-H23 non-small cell lung cancer cells | plasmid DNA | Lipofectamine 3000 |
| NCI-H460 human large cell lung cancer cells | plasmid DNA | Lipofectamine 3000 |
| NCI-H460 human large cell lung cancer cells | plasmid DNA | Lipofectamine LTX |
| NIH/3T3 mouse embryonic fibroblasts | plasmid DNA | Lipofectamine LTX |
| Normal human foreskin fibroblasts (NHFF) | plasmid DNA | Lipofectamine LTX |
| NTERA-2 human embryonal carcinoma stem cells | plasmid DNA | Lipofectamine LTX |
| PC-12 rat neuronal cells | plasmid DNA | Lipofectamine LTX |
| PC-3 human prostate adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| plant protoplasts | DNA | Lipofectin |
| Primary mouse neural progenitor cells (mNPC) | plasmid DNA | Lipofectamine LTX |
| RAW 264.7 mouse macrophage cells | plasmid DNA | Lipofectamine LTX |
| Sf9, Sf21 | baculovirus DNA | Cellfectin II |
| SK-BR-3 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine 3000 |
| SK-BR-3 human breast adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| SK-MEL-28 human malignant melanoma cells | plasmid DNA | Lipofectamine LTX |
| SK-OV-3 human ovarian adenocarcinoma cells | plasmid DNA | Lipofectamine LTX |
| stem cells, (hMSCs) | siRNA, Stealth RNAi | Lipofectamine 2000 |
| stem cells, (hPSCs) in Essential 8 Medium | plasmid DNA, mRNA, RNP | Lipofectamine Stem |
| stem cells, (hPSCs) in mTeSR1 Medium on Geltrex matrix | plasmid DNA, mRNA, RNP | Lipofectamine Stem |
| stem cells, (hPSCs) in StemFlex Medium | plasmid DNA, mRNA, RNP | Lipofectamine Stem |
| stem cells, neural | plasmid DNA, mRNA, RNP | Lipofectamine Stem |
| THP-1 human monocytic leukemia cells | plasmid DNA | Lipofectamine LTX |
For Research Use Only. Not for use in diagnostic procedures.
