Many times science can be 1% inspiration, 99% iteration. Part of getting good results is reviewing your less-than-perfect outcomes and figuring out what to try next time. Learn about some of the problems you may encounter, see examples of what it looks like when things go wrong, and get hints for preventing those same problems in the future.
Learn ways to reduce background (or noise)—the nonspecific fluorescent signal coming from ambient light, the microscope, or the experiment components.
When two or more fluorophores are used, light from one fluorophore can “contaminate” the channel of the other. Learn ways to minimize this bleed-through.
For Research Use Only. Not for use in diagnostic procedures.