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Invitrogen
25-Hydroxy Vitamin D3 Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
1.88 ng/mL
Assay range
3.13-200 ng/mL
Sample type/volume
Plasma
50 µL
Tissue Homogenate
50 µL
Serum
50 µL
Cell Lysate
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
25-OH Vitamin D3
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The 25 Hydroxy Vitamin D3 (25 HVD3) ELISA quantitates 25 HVD3 in serum, plasma and other biological fluids.
Principle of the method
The competitive multispecies ELISA research use only kit is designed to quantitatively measure 25-Hydroxy Vitamin D3 (25-HVD3), independent of species . A 25-HVD3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to 25-Hydroxy Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of 25-HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
25-OH Vitamin D3 (calcifediol) is a prohormone produced by the liver by hydroxylation of vitamin D3 and is used to measure vitamin D status and detect itamin D deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 25-hydroxy vitamin D, 25-hydroxyvitamin D, 25-OH Vitamin D
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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