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Invitrogen
In-Cell ELISA Kit, Colorimetric
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In-Cell ELISA Kit, Colorimetric
A594, A431 and HeLa cells were treated with or without 100 ng/mL EGF and then the levels of pEGFR, pERK and tubulin were measured using the In-Cell ELISA Colorimetric Detection Kit. Results demonstrate that EGF increases the levels of phosphorylated EGFR.
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Product Specifications
Sample type/volume
Supernatant
10,000 cells
Hands-on time
1 hr 20 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
4 x 96 Tests
Contents
Blocking Buffer
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
HRP Conjugate
TMB Substrate
TMB Stop Solution
Janus Green Whole-Cell Stain
Elution Buffer
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
HRP Conjugate
TMB Substrate
TMB Stop Solution
Janus Green Whole-Cell Stain
Elution Buffer
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Intracellular Proteins
Target site-specificity
Protein family
Other Proteins
Assay kit format
In-cell ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The In-Cell ELISA Colorimetric Detection Kit is a simple method for quantifying intracellular proteins in whole cells.
Principle of the method
To perform the assay, cells are first plated, treated and fixed. Expression of the protein(s) of interest is monitored in wells of a microplate using target-specific primary antibodies (see the Important Product Information section for antibodies included in each kit) and a horseradish peroxidase (HRP)-conjugated detection reagent. The kit is supplied with a whole-cell stain to control for differences in cell plating, which is important when measuring relative levels of a protein with different treatments or assessing its post-translational modification (PTM) form. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences among the wells.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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