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Invitrogen
Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit
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![Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit (85-86113-11)](https://www.thermofisher.com/antibody/images/650/TGR.ELISA.Kit-650x600.jpg?time=20220826 "Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit (85-86113-11)")
![Human STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne™ ELISA Kit (85-86113-11)](https://www.thermofisher.com/antibody/images/150/TGR.ELISA.Kit-650x600.jpg?time=20220826)
Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
STAT5 alpha
Target site-specificity
Phosphorylated Tyr694, Phosphorylated Tyr699
Target sub-specificity
Total
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human STAT5A/B (Tyr694/Tyr699) in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : MGF, STAT5, STAT5A
Gene ID : (Human) 6776
Gene symbol : STAT5A
Protein Aliases : Signal transducer and activator of transcription 5A, STAT, STAT5B
UniProt ID (Human) P42229
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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