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Invitrogen
Human Myoglobin ELISA Kit
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Product Specifications
Analytical sensitivity
0.039 ng/mL
Assay range
0.39-25 ng/mL
Sample type/volume
Serum
10 µL
Plasma
10 µL
Cell Lysate
10 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
3.9%
Intraassay CV
5.7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Myoglobin
Target site-specificity
Target sub-specificity
Soluble
Protein subtype
Adaptor Proteins
Protein family
Muscle Protein
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Myoglobin Human ELISA Kit quantitates Hu Myoglobin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Myoglobin.
Principle of the method
The Human Myoglobin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a hemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Mb, MB, PVALB, myoglobgin
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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