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Invitrogen
Human FABP-3 ELISA Kit
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Product Specifications
Analytical sensitivity
26.88 pg/mL
Assay range
156.3-5,000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
6.2%
Intraassay CV
3.9%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard (2 vials)
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard (2 vials)
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
FABP3
Protein subtype
Adaptor Proteins
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human FABP-3 ELISA quantitates Hu FABP-3 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP-3.
Principle of the method
The Human FABP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Fatty acid binding proteins (FABP) are small (approximately 13-14 kDa) intracellular proteins with a high degree of tissue specificity. FABPs are a class of cytoplasmic proteins that bind long chain fatty acids. They are abundantly present in various cell types and seem to play an important role in the intracellular utilization of fatty acids. There are at least six distinct types of FABP, each showing a specific pattern of tissue expression. FABP leaks, due to its small size, rapidly out of is chemically damaged dying cells leading to a rise in serum levels. Liver FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo. Is chemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Next to this L FABP is a marker for rapid hepatocyte lysis in vitro (as for example in toxicology assays) and for detection of liver damage during and after transplantation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FABP11, FABP3, H-FABP, M-FABP, MDGI, O-FABP
Gene ID : (Human) 2170
Gene symbol : FABP3
Protein Aliases : fatty acid binding protein 11, fatty acid binding protein 3, muscle and heart, Fatty acid-binding protein 3, Fatty acid-binding protein, heart, Heart-type fatty acid-binding protein, H-FABP, Mammary-derived growth inhibitor, MDGI, Muscle fatty acid-binding protein, M-FABP, 422 protein, Cardiac Fatty Acid Binding Protein, FABP 3, Fatty acid binding protein 11 (FABP11), Fatty acid binding protein heart, Muscle O-FABP, Mylein protein P2 homolog / adopocyte, heart FABP, Heart type fatty acid binding protein, M FABP, Mammary derived growth inhibitor, muscle and heart (mammary derived growth inhibitor), Muscle fatty acid binding protein
UniProt ID (Human) P05413
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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