Search
Invitrogen
Human CD40L (Soluble) Matched Antibody Pair
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Specifications
Analytical sensitivity
0.16 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Plasma
20 µL
Serum
20 µL
Supernatant
20 µL
Hands-on time
24 hr
Time-to-result
2 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
Sample Diluent
Detection Antibody
Standard
Sample Diluent
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CD154 (CD40 Ligand)
Target site-specificity
Target sub-specificity
Soluble
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human CD40L Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human CD40L. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support