Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.065 ng/mL

Assay range

0.065-16 ng/mL

Sample type/volume

Serum
50 µL
Plasma
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

SCF

Species (tested)

Dog

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Canine SCF/KITLG quantitates canine SCF/KITLG in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant canine SCF/KITLG.

Target information

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : 710-712, CSF, KITLG, MGF

Gene ID : (Dog) 403507

Gene symbol : KITLG

Protein Aliases : c-Kit ligand, Kit ligand, Mast cell growth factor, MGF, SCF, Stem cell factor, stem cell factor, CSF

UniProt ID (Dog) Q06220

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