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Invitrogen
Creatinine Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.75 µg/mL
Assay range
1.25-80 µg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Tissue Homogenate
50 µL
Cell Lysate
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Creatinine
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Creatinine (Cr) ELISA quantitates Cr in serum, plasma and other biological fluids.
Principle of the method
The Competitive multispecies ELISA research use only kit is designed to quantitatively measure Creatinine (Cr) independent of species. A Cr standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Cr is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Cr in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Creatinine is a nitrogenous waste molecule generated from muscle metabolism and is predominantly filtered through the kidneys to be excreted in urine. Although not directly encoded by a gene itself, its levels are significant for assessing kidney function, with serum creatinine being a key biomarker in nephrology. Creatinine evaluation serves as a primary tool for estimating the glomerular filtration rate (eGFR), offering an indirect measure of renal performance. Despite its prevalence in medical diagnostics, creatinine measurement has notable limitations due to its susceptibility to variations influenced by factors such as muscle mass, diet, age, and ethnicity, making it less reliable under certain conditions. Advanced methods now incorporate cystatin C alongside creatinine to enhance equation accuracy for eGFR estimations and mitigate racial biases in clinical settings. The metabolism of creatinine and its physiological significance underscores its utility as a cost-effective, accessible measure for kidney function, despite its shortcomings.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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