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Invitrogen
Dihydrotestosterone Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
23.44 pg/mL
Assay range
39.06-2,500 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Dihydrotestosterone
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Dihydrotestosterone Competitive ELISA Kit quantifies Dihydrotestosterone in serum and plasma.
The Dihydrotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure DHT. A DHT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to DHT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve..
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Dihydrotestosterone (DHT) is a biologically active metabolite of the hormone testosterone, formed primarily in the prostate gland, testes, hair follicles, and adrenal glands by the enzyme 5-alpha-reductase by means of reducing the alpha 4, 5 double-bond. Dihydrotestosterone belongs to the class of compounds called androgens, also commonly called androgenic hormones or testoids. DHT is thought to be approximately 30 times more potent than testosterone because of increased affinity to the androgen receptor. [National Center for Biotechnology Information. PubChem Compound Database; CID=10635].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Andractim, Androlone, Androstanolone, C19H30O2, DHT, STANOLONE
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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